丙型肝炎病毒基因分型与肝病程度关系的研究  

Study on Hepatitis C Virus Genotype in Dalian

作　　者：解莹[1] 谢晨[1] Xie Ying Xie Chen （Department of Infectious Disease of The Second Affiliated Hospital of Dalian Medical University, Dalian liaoning 116027）

机构地区：[1]辽宁省大连医科大学附属二院传染科,辽宁大连116027

出　　处：《中国保健》2010年第10期7-8,共2页

摘　　要：目的探索丙型肝炎病毒（HCV）基因型在辽宁省大连地区的分布及流行的优势型，并探讨HCV基因型与肝病程度的关系。方法采用型特异性引物逆转录巢式PCR法和限制性片段长度多态性分析（RFLP）法对85例抗-HCV阳性的慢性丙型肝炎、丙肝肝硬化、丙肝相关肝癌病人进行HCV的基因分型。结果85份抗HCV阳性标本中，HCVRNA检出率为61例，占71．76％（61／85）。在85份抗HCV阳性血清中，仅检出了61例1b和2a型感染者，其中1b型32例，占52％（32／61）；2a型29例，占48％（29／61）。结论大连地区基因型为1b型和2a型两种型，符合中国丙肝病毒基因分型，大连地区1b型和2a型基本相等。未发现1b和2a基因型因肝病严重程度而有所不同。Objective In order to find out the distribution and prevalence of HCV genotype in Dalian, Liaoning province, and make a research on the connection of HCV genotype and the degree of liver disease. Methods To process with HeY gene classification to the 85 cases which have anti-HeY positive chronic hepatitis C hepatitis C cirrhosis, hepatitis C cancer respectively by means of RT-nested-PCR with primere deduced from 5 i non-coding region and restriction fragment length polymorphism. Results In the 85 anti-HCV positive cases, detection ratio of HCV RNA are 61cases, which account for 71.76% （61/85）. In those 85 anti-HCV positive blood serum, 61cases are detected with lb and 2a, 32 cases with lb which account for 52% （32/61）, 29 cases with 2a which account for 48% （29/61）. Conclusion There are two HCV genotypes in Dalian, namely, lb and 2a, which matches with HCV genotype in China. In Dalian, lb and 2a are equivalent to each other. It is not discovered that there are any differences between lb and 2a because of the severity degree of hepatitis.

关 键 词：肝炎病毒 丙型 基因分型 型特异性引物 

分 类 号：R373.2[医药卫生—病原生物学;医药卫生—基础医学]