MTT法测定多柔比星和氟尿嘧啶及顺铂对肝癌耐药细胞Bel-7402/ADM的IC_(50)值  

Determination of IC_(50) Values of Doxorobicin,Fluorouracil and Cisplatin on Human Multidrug Resistant Hepatoma Carcinoma Cells Bel-7402/ADM by MTT

作　　者：王姗姗[1] 刘明[1] 刘英[1] 汪选斌[1] 

机构地区：[1]郧阳医学院附属人民医院中药药理实验室,湖北十堰442000

出　　处：《医药导报》2010年第5期579-581,共3页Herald of Medicine

基　　金：湖北省自然科学基金资助项目(基金编号:2007ABA211);湖北省教育厅重点项目(基金编号:D200724009);湖北省卫生厅基金资助项目(基金编号:JX3B50)

摘　　要：目的研究多柔比星(ADM)、氟尿嘧啶(5-Fu)、顺铂(DDP)对人肝癌多药耐药细胞株Bel-7402/ADM的细胞毒作用,为开展逆转肿瘤多药耐药研究提供实验依据。方法以MTT法测定ADM、5-Fu、DDP对Bel-7402/ADM的细胞毒作用,计算每种药物的半数抑制浓度(IC50)。结果ADM、5-Fu、DDP对Bel-7402/ADM的IC50分别为10.00,30.60,11.48μmol.L-1。结论MTT法检测药物细胞毒作用方便快捷,节省试剂;随着化疗药物浓度的增加,药物对细胞的抑制率增加。ObjectiveTo study the anti-tumor effect of doxorobicin（ADM）, fluorouracil（5-FU） and cisplatin（DDP） on human multi-drug resistant hepatoma carcinoma cells Bel-7402/ADM, providing basic data for further study. MethodsThe anti-tumor effect of ADM, 5-Fu and DDP on Bel-7402/ADM cells was analyzed by MTT, and IC50 value of each drug was calculated by MTT. ResultsIC50 values of ADM, 5-Fu and DDP for the Bel-7402/ADM were 10.00,30.60,11.48 μmol＆#8226;L-1, respectively. ConclusionDetermining cytotoxicity of drug by MTT was convenient and reagent-saving; cells inhibition was dose-dependently increased by drugs.

关 键 词：多柔比星 氟尿嘧啶 顺铂 肝癌细胞 耐多药 Bel-7402/ADM 半数抑制浓度 

分 类 号：R979.1[医药卫生—药品] R955[医药卫生—药学]