胞内钙库对小麦叶锈菌侵染之过敏反应的影响  

Effect of Intracellular Calcium Stores on Hypersensitive Reaction Induced by Wheat Leaf Rust Fungus

作　　者：张蓓[1] 阎爱华[1] 刘刚[1] 刘猛[1] 侯春燕[1] 王冬梅[1] 

机构地区：[1]河北农业大学生命科学学院,河北保定071001

出　　处：《作物学报》2010年第5期833-839,共7页Acta Agronomica Sinica

基　　金：国家自然科学基金项目(30671244);河北省应用基础研究计划重点基础研究项目(08965505D);河北省自然科学基金项目(303180;C2005000220;C2007000515)资助

摘　　要：使用影响胞内Ca2+库和钙通道的药物预注射小麦叶片,观察其对小麦受叶锈菌侵染诱发的过敏反应(HR)的影响。结果表明,对小麦叶片预注射不同浓度的胞内Ca2+螯合剂(BAPTA-AM)后接种叶锈菌小种260,随着注射药物浓度的增高,寄主细胞发生HR的面积逐渐减小。而注射胞内Ca2+激活剂(caffiene)后接种,HR的面积有所增加。进一步用胞内Ca2+通道抑制剂(herapin、RR和8-Br-cADPR)预处理,结果herapin对HR的影响呈浓度依赖型,而RR和8-Br-cADPR对HR没有明显作用。据此提出,胞内Ca2+可能参与小麦抵抗叶锈菌侵染过程中钙信号的形成,且这一过程主要通过IP3途径完成。With injecting drugs to affect intracellular calcium stores and calcium channel in Wheat （Triticum aestivum） leaves, we investigated hypersensitive reaction （HR） changes in wheat leaves invaded by leaf rust （Puccinia triticina）. The results showed that the intracellular calcium chelators （BAPTA-AM） could obviously reduce the area of dying cells caused by HR. The higher the concentration was, the smaller the area of dying cell was. While intracellular calcium activator （caffeine） was injected into wheat leaves, HR could also be detected. The HR area increased with caffeine concentration. Calcium channel blockers （heparin, RR, and 8-Br-cADPR） were separately injected into wheat leaves before inoculation. The results showed that there was a concentration-depended effect on HR with herapin, and no obviously effect with RR and 8-Br-cADPR. Therefore, we deduced that intracellular calcium might involve in the formation of calcium signal transduction through IP3 pathway during infection of wheat leaf rust fungus.

关 键 词：小麦 叶锈菌 CA2+ 过敏性反应 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治]