基因及构建特异性PCR方法检测转基因香石竹  

Gene and Construct-specific Qualitative PCR Detection of Transgenic Carnations

作　　者：贾军伟[1,2] 孙建萍[2] 白蓝[3] 李鹏[2] 潘爱虎[2] 

机构地区：[1]厦门大学生命科学学院,福建厦门361005 [2]上海市农业科学院生物技术研究所,上海市农业遗传育种重点实验室,农业部转基因植物环境安全监督检验测试中心(上海),上海201106 [3]南京农业大学生命科学学院,南京210095

出　　处：《中国农学通报》2010年第12期35-39,共5页Chinese Agricultural Science Bulletin

基　　金：上海市科技兴农重点攻关项目"转基因植物田间试验监测技术体系建立和产品检测方法研究"(沪农科攻字2008第8-9号)

摘　　要：以澳大利亚Florigene公司和日本Suntory公司研发的两种转基因香石竹Moonshade、Moonlite为研究对象,针对内参照基因ANS和外源基因F3’5’H、CHS启动子、D8ter,建立基因特异性定性PCR检测方法。此外,分别在外源MAC启动子和DFR基因上设计PCR引物,开展构建特异性PCR检测,定性PCR方法的检测灵敏度为0.5%。该方法的建立为转基因香石竹的进口检测、中国监管和环境安全评价提供了初步数据。In this study, In view of the endogenous reference gene ANS and the exogenous genes such as F3＂ 5＂H, CHS promoter, D8ter, the gene and construct - specific qualitative PCR detection methods were established for transgenic carnations Moonshade, Moonlite which were developed by Japanese Suntory company and Australian Florigene company. In addition, PCR primers were designed respectively in the exogenous MAC gene promoter and the DFR gene, construct - specific qualitative PCR detection was carried out. The detection sensitivity reached 0.5%. The establishment of this method provides preliminary data for the transgenic carnation import detection, domestic supervision and environmental safety assessment.

关 键 词：转基因 香石竹 基因特异性 构建特异性 PCR检测 

分 类 号：S3[农业科学—农艺学]