一株香蕉枯萎病生防芽胞杆菌的鉴定、生物学特性和抗菌谱研究  

Identification of A Banana Vascular Wilt Bio-control Bacillus Strain and its Biological Characteristics and Antagonistic Spectrum

作　　者：喻国辉[1] 程萍[1] 王燕鹂[1] 陈远凤[1] 陈燕红[1] 黎永坚[1] 

机构地区：[1]珠海市农业科学研究中心,珠海519075

出　　处：《中国农学通报》2010年第12期216-220,共5页Chinese Agricultural Science Bulletin

基　　金：广东省科技厅教育部产学研结合项目"广谱抗真菌芽胞杆菌杀菌剂的研究与中试"(2007B090400083);珠海市产学研结合配套项目"广谱抗真菌芽胞杆菌杀菌剂的研究与中试"(PC20082043);珠海市科技计划项目"尖孢镰刀菌引起西芹香蕉病害的生物防治技术研究"(PC20081078)资助

摘　　要：API生化鉴定系统对生防芽胞杆菌R31进行鉴定,并研究了该菌株的生物学特性和抗菌谱。结果表明:R31菌株被鉴定为枯草芽胞杆菌,生物学特性数据显示该菌株适宜偏酸或偏碱条件生长,偏酸环境的最佳生长pH为5.13,偏碱环境的最佳pH为8.99,最佳碳源为蔗糖,最佳氮源为酵母浸提物,NA培养基测定的生长曲线显示24h内该菌株生长达到稳定期;抗菌谱研究显示该菌株在平板上对包括多株香蕉枯萎病菌在内的多种植物病原镰刀菌具有明显的抑制效果,主要引起病原菌菌丝肿胀畸形,原生质浓缩,以及菌丝破裂原生质外流。The identification, biological characteristics and antagonistic spectrum of Panama disease bio-control Bacillus strain R31 were studied in this paper. R31 strain was identified as Bacillus subtilis based on the API identification system. The biological characteristics researches showed that R31 grew well at weak acid condition or weak alkalinity condition, the optimum pH in these two different conditions were 5.13 or 8.99, respectively. Sucrose was its optimum carbon source while yeast extract was its optimum nitrogen source; it got the stationary growth phase by cultured in NA medium in 24 h. This strain although had a broad antagonistic spectrum on fungi, lots of Fusarium pathogens included different F. oxysporum f. sp. cubense strains were inhibited obviously by R31 strain in petri dish culture, the hypha of fungi became malformation and broken finally with the protoplasm outflow.

关 键 词：枯草芽胞杆菌 鉴定 香蕉枯萎病 生物学特性 抗菌谱 

分 类 号：S436[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]