LTB融合禽流感病毒M2e基因在毕氏酵母中的表达  

Expression and Construction of Yeast Expression Vector of LBM2eHBc+ gene in P.pastoris

作　　者：张国广[1,2] 张辉煌[1] 董美[1] 陈亮[1] 

机构地区：[1]厦门大学生命科学学院细胞生物学和肿瘤细胞工程教育部重点实验室,福建厦门361005 [2]漳州师范学院生物系,福建漳州363000

出　　处：《云南民族大学学报（自然科学版）》2010年第3期193-197,共5页Journal of Yunnan Minzu University:Natural Sciences Edition

基　　金：福建省科技厅重大项目子课题(2006NZ0003-2);福建省教育厅A类科技项目(JA09168)

摘　　要：禽流感病毒一直是家禽和家畜健康养殖的巨大威胁,之前的研究表明,原核系统中表达的LTB和M2eHBc+融合基因的产物能有效诱导免疫动物对禽流感病毒M2e多肽产生特异性的粘膜免疫应答.酵母作为生物反应器应用于生物制品生产具有独特的优点.本研究构建了pPIC9K-LBM2eHBc+酵母表达载体并对酵母GS115进行了遗传转化,甲醇诱导表达结果表明LBM2eHBc+融合基因在酵母细胞中得到表达,表达出的融合蛋白能够被M2抗体识别.Avian influenza virus（AIV） has been a constant threat to the healthy development of livestock and poultry breeding industry.Previous research has shown that the products of LTB and M2eHBc＋ fused gene expressed in prokaryotic cells can effectively induce mice mucosal immune responses against M2e epitope.P.pastoris yeast has a unique advantage as a bioreactor used in the production of biological products.LBM2eHBc＋ fused gene fragment was obtained by PCR,then inserted into pPIC9K plasmid which contains methanol promoter and yeast signal peptide to construct yeast expression vector pPIC9K-LBM2eHBc＋.The recombinant vector was transformed into P.pastoris GS115.The methanol induction result has indicated that the LBM2eHBc＋ fusion gene can be efficiently expressed in GS115 cell and the expressing protein has the specific binding activity to M2 antibody by Western blotting analysis..

关 键 词：禽流感病毒 LTB M2e表位 毕氏酵母 表达 

分 类 号：Q786[生物学—分子生物学]