听源性惊厥增强大鼠星形胶质细胞Cx43基因表达  

AUDIOGENIC SEIZURE ENHANCES Cx43 GENE EXPRESSION OF ASTROCYTE IN RATS

作　　者：张月华[1] 张国荣[1] 单巍松[1] 王朝晖[1] 吴希如[1] 

机构地区：[1]北京医科大学第一医院儿科,北京100034

出　　处：《中国神经科学杂志》1998年第2期113-116,共4页

基　　金：卫生部科学研究基金

摘　　要：星形胶质细胞之间通过缝隙连接形成偶联，其缝隙连接蛋白为Cx43。该缝隙连接对神经元兴奋时释放到细胞外的K＋起空间缓冲作用。本研究以遗传性癫痫易感大鼠P77PMC为模型，采用原位杂交的方法，观察了一次听源性惊厥后脑内星形胶质细胞Cx43基因表达的变化，结果显示，P77PMC大鼠一次听源性惊厥后在大脑皮质、海马Cx43mRNA表达呈时间依赖性增加，从惊厥后2h开始增加，4～8h达高峰，24h仍高于对照。结果表明：惊厥时，皮质和海马星形胶质细胞Cx43基因表达增强，可能有利于星形胶质细胞对神经元兴奋时释放到细胞外的K＋产生空间缓冲作用，以便维持神经辕围的的K＋平衡。Astrocytes in the central nervous system are extensively coupled by gap junctions. Astroglial gap junction protein is connexin 43 (C-c43). in the present study,genetical epilepsy prone rat P77PMC was used as a model. Changes of Cx43 gene expression in the btain were observed by using in situ hybridization after audiogenic seizure. The resultS indicated that after audiogenic seirure in cerebral cortex and hippocampus,Cx43 mRNA expression showed time-dependent increase,Wean to be elevated at 2 b and reached a peak at 4-8 h,at 24 h it was still higher than the control. The resultS also indicated that neuronal activity could enhance Cx43 gene expression or astrocyte and its probable mechanism was discussed.

关 键 词：星形胶质细胞 缝隙连接 神经元 惊厥 

分 类 号：R720.597[医药卫生—急诊医学]