精加压素片段(4～8)增强大鼠海马组织中钙调蛋白基因表达  

ENHANCEMENT OF CALMODULIN EXPRESSION IN RAT HIPPOCAMPUS BY AVP(4～8) ADMINISTRATION

作　　者：乔利亚[1] 杜雨苍[1] 

机构地区：[1]中国科学院上海生物化学研究所,上海200031

出　　处：《中国神经科学杂志》1998年第2期117-120,共4页

基　　金：国家自然科学基金!No.39570157

摘　　要：利用Northerndotblot技术对钙调蛋白（CaM）mRNA转录水平测定观察到：Wistar大鼠在皮下注射AVP（4～8）1h后，酶法所测得的海马组织中游离的CaM蛋白量增至对照组的3．4倍（P＜0．05）；但同一组织中CaMmRNA转录水平与对照组比较无明显增高，给药3h后，CaMmRNA转录水平达到对照组的2．5倍（P＜0．01），同时CaM蛋白量可达对照组的5．8倍（P＜0．01），推测这时的CaM可能有两个来源：一是结合性CaM的游离，一是基因表达水平的增高。Northern dot blot analyses of calmodulin (CaM) expression indicated that in rat hippocampus the level of CaM transcription had no change at 1 k after AVP(4-8) administration (s. c. );simultaneously, enzymatic det.rmination of free CaM protein demonstrated that the increase of CaM was 3. 4-fold (P<0. 05) in comparison with the control. Three hours later after administration, the transcription significantly rose up to a maximum of 2. 5-fold (P<0. 01) and the protein level happened to be 5' 8-fold (P<0. 01) vs the control. It was suggested that the increase or CaM might occur at two levels, i. e., delivery of Cab pool (GAP43) and expression of CaM gene.

关 键 词：精氨加压素 海马 钙调蛋白 核酸杂交 

分 类 号：R338.26[医药卫生—人体生理学]