^(99)Tc^m-N(NOEt)_2在KMB17人胚肺二倍体细胞与不同种类肺癌细胞中的摄取动力学比较  

^(99)Tc^m-N(NOEt)_2 Uptake Kinetics Difference among KMB17 Human Embryonic Lung Diploid Fibroblast and Different Human Lung Cancer Cells

作　　者：马铁昆[1] 曹剑鸣[1] 贾伟[2] 

机构地区：[1]昆明医学院第一附属医院核医学科,昆明650032 [2]昆明医学院第一附属医院临床实验中心,昆明650032

出　　处：《中国肺癌杂志》2010年第4期307-310,共4页Chinese Journal of Lung Cancer

摘　　要：背景与目的PET/CT显像价格昂贵,因此研究适合SPECT/CT的肿瘤显像剂显得尤为重要。99Tcm-N(NOEt)2[99Tcm-氮-二(N-乙基-N-乙氧基二硫代氨基甲酸盐)]可被肺肿瘤细胞等摄取。本文旨在细胞水平对比研究其在KMB17人胚肺二倍体成纤维细胞与多种肺癌细胞中的摄取动力学差异,探讨99Tcm-N(NOEt)2显像对肺肿瘤的鉴别诊断价值。方法在等体积且细胞浓度为1×106/mL的YTMLC个旧人肺鳞癌细胞、SPC-A1人肺腺癌细胞、AGZY低转移人肺腺癌细胞、973人高转移肺腺癌细胞、GLC-82个旧人肺腺癌细胞以及KMB17人胚肺二倍体成纤维细胞的混悬培养液中分别加入等量99Tcm-N(NOEt)2,按300μL精确取样分装于试管中,37oC恒温培养,均分别于5min、15min、30min、45min、60min、75min、90min离心沉淀,测定细胞内放射性计数,计算各时间点各种细胞对99Tcm-N(NOEt)2的摄取百分率。结果6种细胞除SPC-A1细胞与973细胞之间的差异无统计学意义外(LSD-t检验,P=0.838),其余两两之间的差异均有统计学意义(P<0.001);细胞的摄取率大小关系为:973与SPC-A1>YTMLC>GLC-8->AGZY>KMB17;30min-45min时摄取率逐渐趋于平稳,45min摄取率均大于其摄取峰值的96.6%。结论99Tcm-N(NOEt)2显像在肺肿瘤的鉴别诊断中有一定的临床应用价值,且早期显像在30min左右是适合的。Background and objective PET/CT imaging is expensive,so searching the tumor imaging agent for SPECT/CT is necessary.99Tcm-N（NOEt）2 [bis （N-ethoxy-N-ethyl dithiocarbamato） nitrido99Tcm （V）] can be uptaken by lung cancer cells and other cells alike.The aim of this study is to evaluate the distinctive value in lung tumor with 99Tcm-N（NOEt）2,the difference in its uptake kinetics in human embryonic lung diploid fibroblasts KMB17 and several kinds of lung cancer cells lines.Methods Firstly,six different cell culture medium which contained YTMLC Gejiu human lung squamous carcinoma cell,SPC-A1 human lung adenocarcinoma cell,AGZY low metastatic human lung adenocarcinoma,973 high metastatic human lung adenocarcinoma cell,GLC-82 Gejiu human lung adenocarcinoma cell,and KMB17 human embryonic lung diploid fibroblast,respectively with equal cell density of 1×106/mL and the same volume were prepared;secondly,the same radioactive dose of 99Tcm-N（NOEt）2 was added into each sample and then 300 μL mixed sample was taken out respectively and cultured in 37 oC culture box;Finally,5 min,15 min,30 min,45 min,60 min,75 min,90 min after cultivation,centrifuged each cultured sample and determined the intracellular radiocounts of each sample,calculated each cell sample’s uptake rate of 99TcmN（NOEt）2 at different time.Results Statistical difference was found among six cell samples,and the uptake rate sequence from high to low is 973 and SPC-A1YTMLCGLC-82AGZYKMB17 respectively;furthermore,30 min-45 min after culture,the uptake rate reached stability,and the 45 min uptake rate of each sample was higher than its 96.7% uptake peak.Conclusion Based on the results above mentioned,it is supposed that there are discriminative clinical value when using 99Tcm-N（NOEt）2 as a tumor targeting imaging agent,and 30 min or so after injection may be the best imaging time in the early imaging stage.

关 键 词：肺肿瘤 肺癌细胞 人胚肺二倍体细胞KMB17 N(NOET)2 药代动力学 

分 类 号：R734.2[医药卫生—肿瘤]