灭活菌苗免疫的中华倒刺鲃外周血免疫指标的变化  

Changes of immune parameters in periphery blood of Spinibarbus sinensis Bleeker immunized with formalin-killed Aeromonas sobria

作　　者：罗芬[1,2] 张其中[1,3] 冯汉茹[1] 朱成科[1] 袁娟[1] 组超[1] 

机构地区：[1]西南大学生命科学学院,淡水生物生殖与发育教育部重点实验室,三峡库区生态环境教育部重点实验室,水产科学重庆市市级重点实验室,重庆400715 [2]宁德师范高等专科学校生物工程系,福建宁德352100 [3]暨南大学水生生物研究所,广东广州510632

出　　处：《水产学报》2010年第4期626-634,共9页Journal of Fisheries of China

基　　金：重庆市重大科技攻关资助项目(CSTC,2005AB1009);三峡库区生态环境教育部重点实验室资助项目;福建省教育厅科技项目(JB09233);宁德师范高等专科学校教授培养工程资助项目

摘　　要：以温和气单胞菌灭活菌苗为免疫原,平均体重(100±25)g的健康中华倒刺鲃为实验对象,免疫组腹腔注射0.2mL浓度为1.0×108CFU/mL的免疫原,对照组注射等量灭菌生理盐水,分别在单次注射0、1、2、4、7、14、21、28、35d后随机从两组各取6尾实验鱼,尾静脉采血,测定外周血的血细胞数量、白细胞分类计数、吞噬活性、抗体效价和蛋白质含量等免疫指标的变化,第35天活菌攻毒。结果表明:温和气单胞菌灭活菌苗(F-AS)可诱导中华倒刺鲃红细胞和白细胞数量增加,并引起各种白细胞分类百分比变化,提高吞噬活性和抗体效价,血清中总蛋白及球蛋白含量增加,红细胞也具有免疫功能,灭活茵苗的相对免疫保护力达65.21%。免疫早期(第1周)主要是红细胞、单核细胞和中性粒细胞数量明显增加,吞噬细胞的吞噬活性迅速提高,吞噬百分比和吞噬指数第4天达峰值;随后则是淋巴细胞大量增殖,第21天淋巴细胞、抗体效价及球蛋白达峰值。可见灭活菌苗通过促进中华倒刺鲃血细胞增殖、提高吞噬细胞的吞噬活性、产生特异性抗体等方式提高免疫保护力;免疫早期非特异性细胞免疫起重要作用,之后特异性免疫起主要作用。Spinibarbus sinensis Bleeker in the Yangtze River and its tributaries is of great economical value.However,it suffers from serious diseases,particularly a bacterial disease named ＂muscle ulceration＂in intensive aquaculture.In order to effectively control the disease of S.sinensis,it is necessary to clarify the changes of immune parameters of periphery blood in S.sinensis immunized with a kind of dead bacteria.One hundred and eighty healthy fish（S.sinensis） with an average body weight of（100±25） g were divided randomly into immunized and control groups.Each individual was intraperitoneally injected with 0.2 mL 1.0×108 mL formalin-killed Aeromonas sobria in immunized group,and with 0.2 mL 0.65% sterile saline solution in control group.Then,approximate 2 mL of blood was taken from each fish caudal vein on Day 0,1,2,4,7,14,21,28 and 35 post injection to study the changes of immune parameters,such as haematocyte number,differential leucocyte count（DLC）,serum antibody titer,phagocytic activity of phagocytes and serum protein content.The results were as follows：the number of erythrocytes and leucocytes in peripheral blood of immunized S.sinensis increased during the first 4 days post injection and peaked on 4th day,then,gradually decreased to the control level（Fig.1-a 1-b）.The differential leucocyte count（DLC） of the immunized group increased and was significantly（or very significantly） higher than that of control group for monocytes and neutrophils from day 4 to day 7（Fig.2-a 2-b）,and for lymphocytes from the 14th day to 28th day（Fig.2-c）,but lower than that of control group for thrombocytes on day 4 and 21（Fig.2-d）,and was the same level as that of control group for the four kinds of cells at the other sampling times.The phagocytic percentage（PP） and phagocitic index（PI） of phagocytes in immunized S.sinensis were significantly higher than those of the non-immunized fish between day 2 and day 7,and of the maximum value on day 4,but were not significantly different fro

关 键 词：中华倒刺鲃 灭活温和气单胞菌菌苗 血细胞数量 白细胞分类计数 吞噬活性 抗体效价 

分 类 号：S942.5[农业科学—水产养殖]