血红素加氧酶1对猪瘟病毒复制的影响  

Heme oxygenase 1:a novel interaction protein involved in the replication of classical swine fever virus in PK-15 cells

作　　者：史子学[1,2] 杨知[1,2] 曲会[2] 李江南[2] 朱妍[2] 郭焕成[2] 涂长春[2] 

机构地区：[1]吉林大学畜牧兽医学院,吉林长春130062 [2]军事医学科学院军事兽医研究所,吉林长春130062

出　　处：《中国预防兽医学报》2010年第4期245-248,271,共5页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家973项目子课题(2005CB523200);国家科技支撑计划(2006BAD06A03)

摘　　要：本实验研究了猪血红素加氧酶1对猪瘟病毒(CSFV)在PK-15细胞中复制的影响,本研究应用siRNA干扰方法抑制PK-15细胞中血红素加氧酶1表达后再接种CSFV,细胞中CSFV基因组增殖与对照组相比显著下降;感染病毒细胞中和培养上清中CSFV滴度也显著降低。本实验首次报道了干扰细胞中HO-1表达对CSFV细胞中增殖的影响,提出了CSFV与血红素加氧酶1新的相互作用关系。In this study we defined heme oxygenase 1（HO-1） as a novel interaction partner of Classical swine fever virus（CSFV） in PK-15 cells. To test the functional relevance of HO-1 in CSFV replication,we examined viral replication in PK-15 cells where HO-1 expression had been decreased by siRNA methodology. PK-15 cells were treated with an HO-1-specific siRNA（siHO-1） and followed by inoculation with CSFV. HO-1 depletion in the PK-15 cells led to a significant decline in the viral replication and titers of released virions. Cumulatively,our data suggest that HO-1 is essential for the life cycle of CSFV in PK-15 cells.

关 键 词：猪瘟病毒 血红素加氧酶1 病毒/细胞相互作用 复制 

分 类 号：S852.65[农业科学—基础兽医学]