PRRSV NC株ORF3基因的克隆、序列分析及表达  

Prokaryotic expression of Gp3 protein of porcine reproductive and respiratory syndrome virus NC strain

作　　者：罗军荣[1] 胡国良[1] 余群英[1,2] 兰旅涛[1] 

机构地区：[1]江西农业大学动物科技学院,江西南昌330045 [2]江西省铅山县农业局,江西铅山334500

出　　处：《中国预防兽医学报》2010年第4期322-324,共3页Chinese Journal of Preventive Veterinary Medicine

基　　金：科技部农业科技成果转化资金项目(2009GB2C500191);江西农业大学博士科研启动基金

摘　　要：为原核表达猪繁殖与呼吸综合征病毒(PRRSV)的ORF3基因,本研究根据GenBank登录PRRSV美洲株ATCC VR2332的ORF3基因序列,利用Primer 6.0软件设计合成一对特异性引物,经RT-PCR扩增得到了大小为765bp的片段。将扩增的ORF3基因截短为495bp和750bp片段分别克隆于原核表达载体pGEX-KG中,在IPTG的诱导下进行Gp3重组蛋白的截短表达,经western blot检测证实表达的2种截短重组蛋白均具有良好的与抗体的反应活性,从而为进一步研制新型疫苗提供了实验依据。For prokaryotic expression of Gp3 of protein of porcine reproductive and respiratory syndrome virus（PRRSV） ,the ORF3 gene from a PRRSV NC strain was amplified by RT-PCR using primers derived from PRRSV ORF3 gene encoding Gp3 protein of VR2332 strain. A 765 bp gene fragment was obtained and cloned into the prokaryotic expression vector pGEX-KG,The Gp3 recombinant protein was expressed in E.coli cells. Western blot test confirmed that the expressed recombinant protein could specifically react with the antiserum against PRRSV.

关 键 词：PRRSV ORF3 克隆 GP3蛋白 原核表达 

分 类 号：S852.65[农业科学—基础兽医学]