3种奶牛布鲁氏菌病血清学检测方法的贝叶斯评估  

Evaluation of Three Serological Tests for Brucellosis in Cattle Using Bayesian Analysis

作　　者：刘晓民[1] 杨华[1] 高明春[1] 李彦伟[1] 王君伟[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《畜牧兽医学报》2010年第4期449-453,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家科技支撑计划(2007BAD60B02);现代农业产业技术体系建设专项资金资助(nycytx-0303);黑龙江省重大科技计划(GA06B202)

摘　　要：在无"金标准"的情况下,为评估虎红平板凝集试验(RBT)、荧光偏振分析法(FPA)和间接酶联免疫吸附试验(I-ELISA)用于奶牛布鲁氏菌病的抗体检测能力,作者分别以RBT、FPA、I-ELISA3种方法对采自某奶牛场的血清样品进行检测,通过贝叶斯分析法对检测结果进行分析,研究3种方法检测奶牛布鲁氏菌血清抗体的敏感性(Se)和特异性(Sp)。结果显示,RBT、FPA、I-ELISA3种检测方法的敏感性(Se)分别为91.81%、95.92%和95.98%;特异性(Sp)分别为98.80%、86.54%和98.65%。本研究表明,3种血清学检测方法中以I-ELISA的检测能力最好。The objective of our study was to evaluate the diagnostic performance of three serological tests for brucellosis.Serological test results were used to compare the performance of Rose Bengal Test（RBT）,indirect ELISA（I-ELISA）and Fluorescence Polarisation Assay（FPA）in diagnosing brucellosis in cattle.Their performance was evaluated using Bayesian analysis which allows the sensitivity（Se）and specificity（Sp）to be estimated in the absence of a gold standard.The sensitivity of RBT,FPA and I-ELISA were 91.81%,95.92% and 95.98%,respectively;and specificity of RBT,FPA and I-ELISA were 98.80%,86.54% and 98.65%,respectively.The results demonstrated that the I-ELISA performing well in diagnosing brucellosis in cattle.

关 键 词：布鲁氏菌病 虎红平板凝集试验 间接酶联免疫吸附试验 荧光偏振分析法 贝叶斯分析 

分 类 号：S854.43[农业科学—临床兽医学]