周期素依赖性蛋白激酶-5在不同毛色羊驼皮肤中的表达  

Expression of Cyclin Dependent Kinase 5 in Alpaca Skin of Different Hair Colors

作　　者：刘佳[1] 董常生[1] 范瑞文[1] 贺俊平[1] 高磊[1] 董春光[1] 董彦君[1] 

机构地区：[1]山西农业大学动物科技学院,太谷030801

出　　处：《畜牧兽医学报》2010年第4期478-483,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30571070);山西省青年科技研究基金(2008021038-3)

摘　　要：本研究旨在探索周期素依赖性蛋白激酶-5(CDK5)在羊驼皮肤中的表达与功能。以不同毛色的成年羊驼为研究对象,采用免疫组织化学法对CDK5在羊驼皮肤中的表达进行定位和定性研究,并利用实时荧光定量PCR技术分析了不同毛色羊驼皮肤CDK5基因的相对表达量。免疫组织化学结果显示CDK5在羊驼皮肤毛囊的外根鞘和毛球部呈阳性表达,根据光密度值分析得出CDK5在不同毛色羊驼毛囊中的表达差异显著(P<0.05)。实时荧光定量PCR结果显示棕色羊驼中CDK5mRNA相对基因表达量是白色羊驼的1.6245倍。结果提示CDK5可能与羊驼毛色形成相关。This experiment was conducted to explore the expression and function of cyclin dependent kinase 5 in alpaca skin.The whole testes were obtained from adult alpacas of white and brown hair colors.The localization of CDK5 in alpaca skin was examined by immunohistochemistry,and comparative mRNA expression quantity of CDK5 in alpaca skin of different coat colors was analyzed by QRT-PCR.The results showed that the positive signals were found in hair bulb and outer root-sheath of hair follicles in alpacas,the difference of expression was significant between the white and brown alpaca＇s based on the average optical density（P0.05）.The result of QRT-PCR showed that the comparative mRNA expression quantity of CDK5 in brown alpacas was 1.624 5 times than white ones.The findings showed that CDK5 may involved in the regulation of hair color formation.

关 键 词：CDK5 皮肤 毛囊 毛色形成 免疫组织化学 实时荧光定量PCR 羊驼 

分 类 号：S852.16[农业科学—基础兽医学] Q344.13[农业科学—兽医学]