青黛颗粒对溃疡性结肠炎大鼠结肠黏膜MUC2和iNOS基因表达的影响  

Qingdai Granules up-regulate MUC2 mRNA expression but down-regulate iNOS mRNA expression in the colonic mucosa of rats with ulcerative colitis

作　　者：杜立阳[1] 刘清芳[1] 程晓磊[1] 陈铭诗[1] 李鲜明[1] 仇靖[1] 

机构地区：[1]中国医科大学附属第一医院中医科,辽宁省沈阳市110001

出　　处：《世界华人消化杂志》2010年第9期937-941,共5页World Chinese Journal of Digestology

基　　金：辽宁省自然科学基金资助项目;No.20092130~~

摘　　要：目的:观察青黛颗粒对TNBS诱导的溃疡性结肠炎(UC)大鼠结肠黏膜黏蛋白(MUC2)及诱导型一氧化氮合成酶(iNOS)基因表达的影响,探讨其治疗UC的可能作用机制.方法:将54只SD实验大鼠随机分为正常对照组、模型对照组、阳性药物治疗组(SASP,500mg/kg)、青黛颗粒600、900、1200mg/kg治疗组.造模后第3天开始灌胃给药,共给药10d.实验第14天,处死大鼠,剖取其病变结肠组织,比较各组大鼠的DAI积分和CMDI评分,用逆转录聚合酶链反应(RT-PCR)法检测MUC2及iNOS基因的表达.结果:较模型组青黛颗粒900、1200mg/kg治疗组能显著降低实验大鼠DAI积分和CMDI评分,上调结肠组织中MUC2的基因表达(2.06±0.70vs1.24±0.47;2.34±0.86vs1.24±0.47,均P<0.01),且青黛颗粒1200mg/kg治疗组能下调iNOS的基因表达(0.35±0.12vs0.62±0.31,P<0.05).结论:青黛颗粒可能通过上调结肠黏膜MUC2的基因表达并下调iNOS的基因表达而起到抗TNBS诱导的大鼠溃疡性结肠炎的作用.AIM： To investigate the effects of Qingdai Granules （QDG） on the expression of mucin 2 （MUC2） and inducible nitric oxide synthase （iNOS） mRNAs in the colonic mucosa of rats with ulcerative colitis. METHODS： Ulcerative colitis was induced in rats by giving an enema of trinitrobenzene sulphonic acid （TNBS） and ethanol. The experimental animals were randomly divided into six groups： normal group, model group, sulfasalazine （SASP） group （500 mg/kg）, and low-,medium- and high-dose QDG groups （600, 900 and 1 200 mg/kg）. Except the normal group, the other groups were given intragastrically normal saline, SASP, and different concentrations of QDG, respectively, from day 3 after model establishment for 10 d. On day 14, rats were killed to evaluate disease activity index （DAI） and colon mucosa damage index （CMDI）. The expression of MUC2 and iNOS mRNAs in the colonic mucosa of rats was detected by reverse transcription-polymerase chain reaction （RTPCR）. RESULTS： Compared with the model group, the DAI and CMDI in the medium- and high-dose QDG groups significantly decreased, the expression of MUC2 mRNA in the colon of rats in the medium- and high-dose QDG groups was significantly up-regulated （2.06 ± 0.70 vs 1.24 ± 0.47 and 2.34 ± 0.86 vs 1.24 ± 0.47; both P 0.01）, and the expression of iNOS mRNA in the high-dose QDG group was significantly down-regulated （0.35 ± 0.12 vs 0.62 ± 0.31, P 0.05）. CONCLUSION： QDG can exert protective effects against rat ulcerative colitis perhaps by upregulating MUC2 mRNA expression and down-regulating iNOS mRNA expression.

关 键 词：溃疡性结肠炎 青黛颗粒 MUC2 诱导型一氧化氮合成酶 

分 类 号：R285.5[医药卫生—中药学]