甜菜SRAP扩增产物两种不同检测方法的比较  

Comparison of Denatured and non-denatured PAGE-Silver-Staining Detection of SRAP-PCR Product in Beet

作　　者：吴则东[1,2,3,4] 王华忠[1,2,3,4] 韩英[1,2,3,4] 

机构地区：[1]黑龙江省普通高等学校甜菜遗传育种重点实验室,哈尔滨150080 [2]中国农业科学院北方糖料作物资源与利用重点开放实验室,哈尔滨150080 [3]中国农业科学院甜菜研究所 [4]黑龙江大学农作物研究院,哈尔滨150080

出　　处：《中国糖料》2010年第2期9-11,共3页Sugar Crops of China

基　　金：甜菜现代产业技术体系建设-甜菜丰产优质种质资源及新品种选育(nycytx-25-01-05)项目

摘　　要：为了研究不同电泳及染色方法对甜菜SRAP扩增产物的影响,以6个甜菜二倍体品系为材料,比较变性聚丙烯酰胺凝胶电泳与非变性聚丙烯酰胺凝胶电泳对SRAP-PCR扩增产物用不同银染方法的检测效果进行了比较分析。结果显示:非变性聚丙烯酰胺凝胶电泳与变性聚丙烯酰胺凝胶电泳的分辨率差异不大,但非变性聚丙烯酰胺凝胶电泳具有灌胶简单易学,显色时间短,节省药品等特点,可以作为今后甜菜SRAP电泳及显色的首选方法。To test the effect of different electrophoresis and coloration methods on SRAP （sequence-related amplified polymorphism） product, the detection effect experiment of denatured and non-denatured polyacrylamide gel electrophoresis -silver- staining techniques of SRAP-PCR product in beet was conducted. The results showed that the resolving power was not significant differences between denatured and non-denatured polyacrylamide gel electrophoresis （PAGE）. The non-denatured PAGE was characteristics with simple to learn for filling gel, short chromogenic times and medicine saving. It could be used as the first method of SRAP electrophoresis and coloration in sugarbeet.

关 键 词：甜菜 SRAP 电泳方法 银染方法 

分 类 号：S566.301[农业科学—作物学]