西瓜与枯萎病菌非亲和互作的表达序列标签分析  

Analysis of Expressed Sequence Tags in the Incompatible Interaction Between Watermelon and Fusarium oxysporum

作　　者：吕桂云[1,2,3] 郭绍贵[2] 张海英[2] 耿丽华[2] 许勇[2] 

机构地区：[1]中国农业科学院蔬菜花卉研究所,北京100081 [2]国家蔬菜工程技术研究中心,北京100097 [3]河北农业大学园艺学院,河北保定071001

出　　处：《中国农业科学》2010年第9期1883-1894,共12页Scientia Agricultura Sinica

基　　金：国家"十一五"科技支撑计划项目(2006BAD01A7-6-02);国家现代西瓜甜瓜产业技术体系(nycytx-36-01-01-01);国家自然科学基金项目(30972015);北京市自然科学基金项目(5100001);北京市科委项目(Z09090501040902;D08070500690803)

摘　　要：目的从整体水平上阐明西瓜与枯萎病菌非亲和互作基因表达的特征。方法用SSH方法构建枯萎病菌接种处理后不同时间点的cDNA文库,对其中获得3895条高质量的表达序列标签(EST)序列进行分析。结果在测定4431条cDNA序列中,序列拼接后获得1756条unigene,经过注释和分析,发现可能参与西瓜与枯萎病菌非亲和互作的转录因子、激酶和防卫基因,及茉莉酸和木质素生物合成途径等。结论在西瓜与枯萎病菌互作的SSH-cDNA文库中,与抗病防御相关基因约占36.3%,初步了解了西瓜与枯萎病菌非亲和互作过程中基因表达信息,这些基因的发现为在分子水平上研究西瓜与枯萎病菌的互作机制以及相关重要基因的克隆与功能分析奠定基础。Objective The objective of the experiment is to study the mechanisms of watermelon resistance to Fusarium oxysporum by investigating the expression profiles of the related genes in the incompatible interaction. Method A cDNA library of the watermelon root tissues induced by Fusarium oxysporum f. sp. niveurn was constructed using the SMART protocol. The positive clones of the cDNA library were sequenced randomly and 3 895 desired ESTs were obtained for bioinformatics analysis. Result Total 1 756 unique sequences were annotated for the functions. Transcription factors, protein kinase, defence gene, shikimate-phenylpropanoid-lignin metabolism and JA biosynthesis were found in the incompatible interaction between watermelon and Fusarium oxysporum. Conclusion There were 36.3% of the annotated unique sequences had similar function with disease resistance and defense. This information showed us an elementary profile of the interaction mechanism between watermelon and Fusarium oxysporum. These results developed a good foundation for the further exploration of the interaction mechanism at the molecular level and the related important genes cloning.

关 键 词：西瓜 枯萎病菌 CDNA文库 表达序列标签(EST) 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]