大豆异黄酮对去卵巢大鼠脾脏IL-2 mRNA表达的影响  

Effect of Soy Isoflavone on the Expression of IL-2 mRNA in the Ovariectomized Rat’s Spleen

作　　者：陈正礼[1] 罗启慧[1] 程安春[1] 

机构地区：[1]四川农业大学动物医学院/动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《中国农业科学》2010年第9期1948-1953,共6页Scientia Agricultura Sinica

基　　金：四川省科技厅青年基金(08ZQ026-061);教育部"长江学者和创新团队发展计划"创新团队项目(IRT0848)

摘　　要：目的探讨大豆异黄酮对去卵巢大鼠脾脏IL-2mRNA表达及分布的影响。方法在成功造模60只去卵巢青年大鼠的基础上,分别皮下注射补充大豆异黄酮高、中、低剂量(1.5、1.0、0.5mg·kg-1BW)和溶媒剂,补充至第2、4和6周时每组各宰杀5只,运用原位组织杂交方法,对大鼠脾脏IL-2mRNA的表达和分布变化进行观察。结果IL-2mRNA主要分布于被膜下方的红髓区,在白髓区的脾小结外周、边缘区、动脉周围淋巴鞘外层等处也有分布。大鼠去卵巢后脾脏中IL-2mRNA的表达强度和阳性细胞数目均显著下降,并且随着去除卵巢时间的延长更趋明显;而补充大豆异黄酮高、中、低剂量后,各组IL-2mRNA的表达强度和阳性细胞数目随着时间的延长均呈上升趋势,表现时间依赖性。在同一时间段内,随着补充大豆异黄酮剂量的升高,IL-2mRNA的表达强度和阳性细胞数目也均呈上升趋势,表现为剂量依赖性。结论大豆异黄酮对脾脏内IL-2mRNA的表达具有上调作用,并存在剂量和时间依赖性。Objective The objective of the experiment is to research on the effects of soy isoflavone on expression of IL-2 mRNA in the spleen of ovariectomized （OVX） rat. Method Sixty young female rats were ovariectomized, and then these rats were supplied with soy isoflavone respectively in high dosage （1.5 mg·kg-1）, medium dosage （1.0 mg·kg-1）, low dosage （0.5 mg·kg-1） and solvent agent via hypodermic injection. At the second, fourth and sixth week after treatment, 5 rats per group were killed for study. The expression and distribution of IL-2 mRNA in the spleen were studyed by in situ histo-hybridization method. Result The IL-2 mRNA mainly distributed in red pulp close to the capsule, some in periphery of splenic corpuscle, marginal zone and outer layer of periarterial lymphatic sheath. The general tendency of the intensity and the number of IL-2 mRNA positive cells of OVX rats are significantly decreased, and the degression was more significantly with the time going after ovariectomizing treatment. While after treatment with soy isoflavone, the general tendency of the intensity and the number of IL-2 mRNA positive cells of OVX rats were increased and showed a time-dependent manner. In the same period of time, with the increasing concentration of soy isoflavone, the general tendency of the intensity and the number of IL-2 mRNA positive cells were increased and showed a dose-dependent manner. Conclusion The above results suggested that soy isoflavone could upregulate the expression of IL-2 mRNA and showed a time-and dose-dependent manner.

关 键 词：大豆异黄酮 原位组织杂交方法 IL-2MRNA 脾脏 去卵巢 大鼠 

分 类 号：S865.12[农业科学—野生动物驯养]