大孔吸附树脂在瑞拉菌素高产菌GAO-1-GR-2活性物质提取中的应用  

Macroporous Resin in Adsorption Process of an Tifungal Produced by Zuelacmycin-high-yielding Strain GAO-1-GR-2

作　　者：程建坤[1] 侯冬媛[1] 苟丽霞[1] 安德荣[1] 

机构地区：[1]西北农林科技大学植物保护学院陕西省农业分子生物学重点实验室,陕西杨凌712100

出　　处：《农药》2010年第5期334-337,共4页Agrochemicals

基　　金：国家"863项目"资助(2007AA021503)

摘　　要：以瑞拉菌素高产菌GAO-1-GR-2发酵液为基础,选用不同粗提温度和5种不同的大孔吸附树脂提取瑞拉菌素高产菌GAO-1-GR-2抑菌活性物质,以稻瘟菌为指示菌,进行吸附条件优化。结果表明:最佳粗提温度为60℃,GAO-1-GR-2抑菌活性物质活性最强且提取效率最高,HP-20对活性物质的吸附效果较好,并且获得了较优的吸附条件:上样液pH值为6.0,最佳的吸附流速为2.0mL/min,最佳的洗脱液为体积分数50%甲醇、70%乙醇和30%丙酮,洗脱液最佳流速为1.5mL/min。Based on fermentation broth of Zuelacmycin-high-yielding strain GAO-I-GR-2, antifungal active substances were extracted from the fermentation broth on the conditions of different extracting temperatures and five different kinds of macroporous adsorption resins. Pbyricularia grisea was used as indicating microorganism. The results showed that the optimum extracting temperature was 60 ℃ and macroporous adsorption resin HP-20 had better adsorption effect to active substances. After the optimization of absorption conditions, the optimum pH of sample was 6.0, optimum absorption velocity was 2.0 mL/min, the optimum component of eluent was methanol 50%, ethanol 70% and acetone 30%（by vol）, the optimum velocity of eluent was 1.5 mL/min.

关 键 词：瑞拉菌素 大孔树脂 吸附 优化 

分 类 号：TQ450.21[化学工程—农药化工]