皂角刺总黄酮诱导HCT116细胞凋亡的透射电镜观察  

ELECTRON MICROSCOPIC OBSERVATIONS ON THE APOPTOSIS OF HCT116 CELL LINE INDUCED BY FLAVONE COMPONENTS FROM SPINA GLEDITSIAE

作　　者：肖顺汉[1] 姚健[2] 刘明华[1] 李荣[1] 任美萍[1] 李蓉[1] 

机构地区：[1]泸州医学院药理教研室 [2]泸州市人民医院外科,四川泸州646000

出　　处：《泸州医学院学报》2010年第2期116-118,共3页Journal of Luzhou Medical College

基　　金：四川省教育厅重点项目(2005A076)

摘　　要：目的:研究皂角刺总黄酮对结肠癌HCT116细胞增殖和凋亡的影响。方法:应用CCK-8试剂盒检测对细胞增殖的抑制作用,透射电镜观察对细胞凋亡的超微结构变化。结果:皂角刺总黄酮能够显著抑制HCT116细胞的增殖,且呈现出一定的剂量依赖性。皂角刺总黄酮作用48小时后,HCT116细胞透射电镜可见核固缩、碎裂和凋亡小体等凋亡形态学改变。结论:皂角刺总黄酮可抑制结肠癌细胞HCT116的增殖,诱导其凋亡。Objective：To study the apoptosis of Colon cancer HCT116 cell line induced by Flavone Components from Spina Gleditsiae.Methods：The inhibition of cell proliferation was determined by CCK-8（Cell Counting Kit-8） assay.The morphological changes of the apoptosis cells were observed by transmission electron microscope.Results：Flavone Components from Spina Gleditsiae significantly inhibited the proliferation of HCT116 cells in a dose-dependent manner.Colon cancer cells apoposis was morphologically observed after 48hours induction by Flavone Components from Spina Gleditsiae.Conclusion：Flavone Components from Spina Gleditsiae can inhibit the proliferation of HCT116 cells line and induce their apoptosis.

关 键 词：皂角刺总黄酮 HCT116细胞 凋亡 电镜 

分 类 号：R285.5[医药卫生—中药学]