高密度脂蛋白和低密度脂蛋白对HepG2细胞SR-BI表达的影响  

Influences of the SR-BI Expression by HDL,LDL,and ox-LDL in HepG2 Cells

作　　者：莫中成[1] 陈欣[2] 曹轩[3] 唐显庆[1] 石金凤[1] 龙治峰[1] 易光辉[2] 

机构地区：[1]南华大学医学院组织胚胎学教研室,湖南衡阳421001 [2]南华大学心血管病研究所 [3]南华大学药物药理研究所

出　　处：《南华大学学报（医学版）》2010年第1期10-13,20,共5页Journal of Nanhua University(Medical Edition)

基　　金：湖南省教育厅高校重点科研项目(09A078)

摘　　要：目的观察高密度脂蛋白(HDL)、低密度脂蛋白(LDL)及氧化型低密度脂蛋白(ox-LDL)对HepG2细胞B类I型清道夫受体(SR-BI)表达及胆固醇含量的影响。方法用HDL、LDL及ox-LDL处理HepG2细胞,采用RT-PCR、Western印迹检测细胞SR-BI的表达;采用油红O染色及高效液相色谱法(HPLC)观察细胞内脂质的含量。结果HDL、LDL及ox-LDL处理HepG2细胞后,与对照组比较,各脂蛋白处理组细胞SR-BI mRNA及蛋白表达上调,油红O染色发现HDL、LDL及ox-LDL处理组细胞内脂滴含量明显增加;HPLC检测提示,与对照组细胞内总胆固醇含量(43.18±7.80 mg/g蛋白)比较,ox-LDL处理组(113.01±15.90 mg/g蛋白)、LDL处理组(171.13±20.01 mg/g蛋白)及HDL处理组(220.39±28.00 mg/g蛋白)细胞内总胆固醇含量增加,其中HDL处理组变化最明显。结论HDL、LDL及ox-LDL三种脂蛋白可上调HepG2细胞SR-BI的表达,增加细胞内胆固醇的蓄积。Objective To investigate the influences of the SR-BI expression and cholesterol content by ox-LDL,LDL and HDL in HepG2 cell lines. Methods HepG2 cells were co-incubated with 50 μg/mL ox-LDL,LDL and HDL,respectively.SR-BI mRNA and protein level were determined by reverse transcription-polymerase chain reaction and western blot.Cellular cholesterol content was determined by Oil Red O staining and high performance liquid chromatography analysis. Results Ox-LDL,LDL and HDL up-regulated the expression of SR-BI in HepG2 cells compared with control group by RT-PCR and Western blot.At the same time,lipid droplets were increased in ox-LDL,LDL and HDL-treatment groups by Oil Red O staining.Total cholesetro content of HepG2 cells was increased in ox-LDL（113.01±15.90 mg/g protein）,LDL（171.13±20.01 mg/g protein） and HDL（220.39±28.00 mg/g protein） compared with control group（43.18±7.80 mg/g protein）. Conclusions Our results indicated that ox-LDL,LDL and HDL up-regulated the SR-BI expression and increased cellular cholesterol content in HepG2 cells.

关 键 词：B类I型清道夫受体 脂蛋白 胆固醇 

分 类 号：R541.4[医药卫生—心血管疾病]