LGSF-MTs体系的共振散射光谱及分析应用  

Resonance Light Scattering Spectrophotometric Study of Light Green SF(Yellow) With Metallothioneins and Its Analytical Application

作　　者：薛金花[1] 王永生[1] 何爱桃[1] 郝玉娥[1] 

机构地区：[1]南华大学公共卫生学院,湖南衡阳421001

出　　处：《南华大学学报（医学版）》2010年第1期31-33,共3页Journal of Nanhua University(Medical Edition)

基　　金：湖南省自然科学基金资助项目(03JJY3030);衡阳市基础研究计划项目(2009kJ01)

摘　　要：目的建立LGSF-RLS增强定量检测尿样中金属硫蛋白（MTs）分析的新方法。方法在pH2.5 BR缓冲溶液中,亮绿SF淡黄（LGSF）与MTs作用,导致体系的共振光散射（RLS）明显增强。结果LGSF-MTs体系在468 nm波长处的RLS增强值（ΔI）与MTs的浓度（ρMTs）有良好的线性关系,线性范围0.05-5.00μg/mL,相关系数为0.9992,方法检出限15.50 ng/mL,相对标准偏差1.01%-5.15%（n=9）,平均加标回收率94.7%。结论该方法最突出的特点是灵敏、准确、易于推广,用于样品测定结果较准确。Objective A novel quantitative method was developed for the determination of metallothioneins（MTs） using light green SF（yellow） by technology of RLS. Methods In the Britton-Robinson buffer solution（pH2.5）.Results The enhancement of the system at 468nm was proportional to the concentration of MTs in the range of 0.05-5.00 μg/mL,with the detection limit of 15.50 ng/mL,the RSD of 1.01%～5.15%（n=9）,and the average recovery of 94.7%. Conclusion The method was sensitive and simple apply in the detection of MTs with satisfaction.

关 键 词：亮绿SF淡黄 金属硫蛋白 生物标志物 共振光散射 

分 类 号：R12[医药卫生—环境卫生学]