HO-1介导H_2O_2预处理对PC12细胞氧化应激损伤的适应性细胞保护作用  

Heme Oxygenase-1 Mediates H_2O_2 Preconditioning-induced Adaptive Cytoprotection Against Oxidative Stress in PC12 Cells

作　　者：唐小卿[1] 陈容前[1] 李玉娟[1] 何剑琴[1] 尹蔚兰[1] 胡弼[1] 冯鉴强[2] 

机构地区：[1]南华大学医学院生理学教研室,湖南衡阳421001 [2]中山大学中山医学院生理学教研室

出　　处：《南华大学学报（医学版）》2010年第2期159-162,共4页Journal of Nanhua University(Medical Edition)

基　　金：湖南省教育厅资助课题(No.06C700);湖南省科技计划项目(No.05FJ3039)

摘　　要：目的探讨血红素氧合酶-1(HO-1)对H2O2预处理的适应性细胞保护效应的介导作用。方法PI染色流式细胞仪(Flowcytometer,FCM)检测PC12细胞凋亡,流式细胞仪检测PC12细胞HO-1蛋白的表达。结果PC12细胞经10μmol/L H2O2预处理90 min后,20、30、50、100μmol/L H2O2对PC12细胞凋亡的诱导作用明显下降;10μmol/L H2O2预处理90 min后,PC12细胞HO-1蛋白表达量明显增加;给予10μmol/L的HO-1蛋白特异性阻断剂ZnPP后,可显著阻断10μmol/L H2O2预处理90 min对50μmol/L H2O2损伤PC12细胞的保护作用。结论血红素氧合酶-1(HO-1)介导了H2O2预处理对PC12细胞氧化应激损伤的适应性细胞保护作用。Objective To investigate whether heme oxygenase - 1 （ HO - 1 ） mediate the adaptive cytoprotection of H2O2 preconditioning against oxidative stress in PC12 cells. Methods Apoptosis of PC12 cells was detected by flow cytometer （FCM） after PI staining and the expression of HO - 1 in PC12 ceils was determined by FCM assay. Results Pretreatment with 10 μmol/L H2O2 for 90 rain inhibited the apoptosis of PC12 ceils induced by H2O2 from 20 μmol/L to100 μmol/L. The expression of HO - 1 in PC12 cells was up - regulated by pretreatment with 10 μmol/L H2O2 for 90 min. ZnPP, a special inhibitor of HO - 1, blocked the protective effect of pretreatment with 10 μmol/L H2O2 for 90 min on the apoptosis of PC12 cells induced by 50 μmol/L H2O2. Conclusion HO - 1 mediates the adaptive cytoprotection of H2O2 preconditioning against oxidative stress in PC12 cells.

关 键 词：血红素氧合酶-1 H2O2 预处理 氧化应激 适应性细胞保护 

分 类 号：R33[医药卫生—人体生理学]