柠檬酸镧对肝癌细胞HepG2抗失巢凋亡的影响  

Effect of lanthanum citrate on anoikis resistance in human hepatoma cells HepG2

作　　者：苏湘鄂[1] 郑小娜[1] 

机构地区：[1]深圳大学生命科学学院,深圳518060

出　　处：《深圳大学学报（理工版）》2010年第2期236-240,共5页Journal of Shenzhen University(Science and Engineering)

基　　金：国家自然科学基金重点资助项目(20607010)

摘　　要：研究柠檬酸镧对肝癌细胞HepG2失巢凋亡的影响.采用聚羟乙基异丁烯酸阻断细胞与胞外基质联系的方法,建立体外细胞失巢生长模型.在贴壁和失巢条件下,用浓度0.1、0.01和0.001 mmol/L的柠檬酸镧溶液处理细胞.对于贴壁细胞,用碘化丙啶(propidium iodide,PI)染色检测细胞周期和凋亡率.对于失巢细胞,运用AnnexinV FITC-PI双染法检测失巢凋亡,用线粒体膜电位检测试剂盒JC-1(5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethyl-imidacarbocyanine iodide)荧光显微镜法检测线粒体膜电位.结果表明,在贴壁条件下,柠檬酸镧处理组的周期和凋亡率与对照组相比,不存在明显差异.而在失巢条件下,柠檬酸镧促进HepG2细胞失巢凋亡,降低了线粒体膜电位.结果显示,稀土元素可能具有作为新的抗癌药物的潜力.Effect of lanthanum citrate on the anoikis of human hepatoma （HepG2） cells was investigated in this study. Polyhydroxyethylmethacrylate （Poly-HEMA） was used to establish the non-attachment culture model by cell- contact blocking. HepG2 cells were treated with 0. 1, 0.01 and 0. 001 mmol/L lanthanum citrate under attachment condition and non-attachment condition. Cell cycles and apoptosis of HepG2 under attachment condition were detected by PI （propidium iodide） staining. Anoikis and mitochondrial membrane potential of HepG2 under non-attachment condition was measured by Annexin V-PI double staining and JC-1 （5, 5＇, 6, 6＇-tetrachloro-1, 1＇, 3, 3＇- tetraethyl-imidacarbocyanine iodide） fluorescence microscope method respectively. Results showed that lanthanum citrate treatment brought no difference in cell cycle and anoikis to HepG2 under attachment condition but increased the anoikis and decreased mitochondrial membrane potential of HepG2 under non-attachment condition. These results implied that rare earth elements may have the potential as a new cancer treatment medicine.

关 键 词：细胞生物学 细胞死亡 稀土元素 柠檬酸镧 失巢凋亡 肝癌细胞 HEPG2细胞 线粒体膜电位 

分 类 号：Q279[生物学—细胞生物学] O614.331[理学—无机化学]