溶血磷脂酸对人肺成纤维细胞Ⅰ型胶原合成的影响  

The Effect of Lysophosphatidic Acid on Procollagen Type Ⅰ Gene Expression in Human Lung Fibroblasts

作　　者：夏小春[1] 李文林[2] 石小玉[3] 何晓璐[3] 陈雄林[4] 赵林[2] 

机构地区：[1]南昌大学研究生院医学部 [2]江西省医学科学研究所,南昌330006 [3]南昌大学医学院组织学与胚胎学教研室 [4]九江学院组织学与胚胎学教研室,江西九江332000

出　　处：《南昌大学学报（医学版）》2010年第1期1-4,共4页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(30860118);江西省卫生厅科技课题(20062016;20072017);江西省教育厅科技课题[赣教技字(2007)81号]

摘　　要：目的探讨溶血磷脂酸(lysophosphatidic acid,LPA)对人肺成纤维细胞(HFL-1)Ⅰ型胶原(procollagen typeⅠ,PCOLⅠ)合成的影响。方法将HFL-1培养后置于6孔板中,随机分为6组:对照组,LPAⅠ、Ⅱ、Ⅲ组(用1μmol.L-1的LPA刺激6、12、24h后收集细胞),IL-13组(用100μg.L-1的IL-13作用48h后收集细胞),LPA+IL-13组(用1μmol.L-1的LPA刺激12h、100μg.L-1的IL-13刺激48h后收集细胞),每组3孔。用RT-PCR方法检测PCOLⅠmRNA的表达;用免疫组化方法检测PCOLⅠ蛋白的表达。结果免疫组化与RT-PCR方法均显示LPA刺激后,PCOLⅠ的表达降低,IL-13刺激后,PCOLⅠ的表达增高,且LPA+IL-13组PCOLⅠ表达比LPA组较高,比IL-13组较低。结论LPA可以下调HFL-1PCOLⅠ的合成。Objective To investigate the effect of lysophosphatidic acid （LPA） on procollagen type I （PCOL I ） gene expression in human lung fibroblasts（HFL-1）. Methods HFL-1 cultured and then seeded 6-well plates,and then divided into 6 groups：control group, LPA I . II .III group（cells were stimulated with 1 umol . L- 1 LPA 6,12,24 h respectively,and then collected）, IL-13 group （cells were stimulated with 100 ug . L-1 IL-13 48 h and then collected） ,LPA＋IL-13 group（cells were stimulated with 1 umol . L-1 LPA 12 h and 100 ug . L-1 IL-13 48 h,then collected）,cells were cultured in 3 wells each group. The expression of PCOL I mRNA was evaluated by RT-PCR. The expression of PCOL I protein was evaluated by immunohistochemisty. Results The results of RT-PCR and immunohistochemisty both demonstrated that LPA could reduce the expression of PCOL I in HFL-1. In addition, the expression of PCOL I in IL-13 group was higher than that in control group,and IL-13＋LPA group was higher than that in LPA group but lower than IL-13 group. Conclusion The expression of PCOL I is down-regulated when HFL-1 cells are stimulated with LPA.

关 键 词：溶血磷脂酸 人肺成纤维细胞 白介素13 Ⅰ型胶原 

分 类 号：R392.12[医药卫生—免疫学]