RP-HPLC法同时测定人血清咪哒唑仑、阿普唑仑与氯硝安定药物浓度  

Simultaneous Measurement of Human Serum Concentration of Midazolam, Alprazolam and Clonazepam with RP-HPLC

作　　者：吴学军[1] 

机构地区：[1]湖南省脑科医院药剂科,湖南长沙410015

出　　处：《医学临床研究》2010年第4期653-655,共3页Journal of Clinical Research

摘　　要：【目的】建立一高效液相色谱法同时测定人血清中咪哒唑仑（MZ）、阿普唑仑（AZ）、氯硝安定（CZ）药物浓度，为临床用药及治疗提供依据。【方法】采用反相高效液相色谱法，色谱柱为依利特Kromasil C18柱（250mm×4．6mm，5μm）；柱温40℃；流动相为甲醇-水（65：35，v／v），流速1．0mL／min；波长220nm。血液样品采用10％高氯酸沉淀蛋白后，直接进样20μL，测定，采用内标法定量。【结果】MDZ、AZ、CZ在0．025～10．00μg／mL浓度范围内线性关系好，相关系数分别为r=0．9994，0．9993，0．9991，最低检测限均为5．0ng／mL，三种物质的平均方法回收率均大于92．0％，相对标准偏差（RSD）〈4．1％。【结论】本法测定MZ、AZ、CZ的血药浓度方法简便、快速准确，可用于临床的常规监测。[Objective] To develop a reverse phase high performance liquid chromatography（RP HPLC） for simultaneous determination of human serum concentration of midazolam（MZ）, alprazolam（AZ） and clonazepam（CZ） and monitor the useage of these compounds. [Methods] Kromasil C18 column was used and the col umn temperature was 40 ℃. The mobile phase was methanol/water （65：35, V/V）. The flow rate was 1.0 mL/min. The detection wave length was 220 nm. Twenty μL sample was injected after the plasma protein was precipitated with10% perchloric acid. Internal standard was used for quantitation. [Results] The linear relation of MZ, AZ and CZ was in the range of 0. 025-10.00 μg/mL. The minimum detection limit of MZ, AZ and CZ was 5.0 ng/mL. The average coefficient of recovery of three drugs was more than 92.0% and the relative standard deviation（RSD） was less than 4. 1%. [Conclusion]RP-HPLC method is a rapid, accurate and simple assay to measure MZ, AZ and CZ concentration in human serum and it can be applied routinely to monitor the concentration of these three drugs.

关 键 词：咪达唑仑/分析 阿普唑仑/分析 氯硝西泮/分析 色谱法 高压液相 

分 类 号：R971.4[医药卫生—药品]