鼻息肉中核因子кB的活化对IL-1a转录的影响及意义  

Effects of NF-кB activation on the transcription of IL-1a in tissues of nasal polyps

作　　者：王朝永[1] 张麟燕[1] 赵瑜[1] 王平[1] 廖秀清[1] 谢才毅[1] 熊仁平[2] 

机构地区：[1]重庆市涪陵中心医院,重庆涪陵408000 [2]第三军医大学附属大坪医院野战外科研究所,重庆400042

出　　处：《西部医学》2010年第5期808-810,共3页Medical Journal of West China

基　　金：重庆市涪陵区科技计划项目(No:2005-1-6)

摘　　要：目的探讨鼻息肉组织中核因子кB(Nucler factorкB,NF-кB)的活化对IL-1a转录的影响及意义。方法取32例确诊为鼻息肉患者的息肉组织,以其中的14例患者的鼻中隔黏膜作对照,用凝胶迁移实验(Electrophoreticmobility shift assay,EMSA)检测NF-кB的DNA结合活性,用RT-PCR检测IL-1a mRNA的表达,对NF-кB的DNA结合活性与IL-1a的转录水平行Pearson相关性分析。结果鼻息肉组织中NF-кB的DNA结合活性升高,明显高于对照组(p<0.01);鼻息肉组织中IL-1a mRNA的表达明显高于对照组(P<0.01);Pearson相关性分析提示,鼻息肉组织中NF-κB的DNA结合活性与IL-1a的转录呈显著正相关(r=0.777,P<0.01)。结论IL-1a是鼻息肉中NF-кB激活后所分泌的重要的细胞因子,NF-кB和IL-1a引起的局部微环境的改变可能是鼻息肉发生的重要内在因素。Objective To investigate the effects of NF-κB activation on transcription of IL-1a in the tissues of nasal polyps. Methods The specimens from 32 nasal polyps and 14 nasal septum mucous membranes were studied by electrophoretic mobility shift assay（EMSA）for the activation of NF-κB and by reverse-transcription polymerase chain reaction （RT-PCR） for the expression of IL-1a mRNA. The correlations between the activation of NF-κB and the transcription of IL-1a mRNA were evaluated by Pearson correlation analysis. Results The activation of NF-κB DNA were significantly increased in the tissues of nasal polyps compared that of nasal septum mucous membranes （P〈0.01）. The expression of IL-1a mRNA were significantly increased in nasal polyps than that in controls （P〈0.01）. The Pearson correlation analysis demonstrated that there were positive relationship between the activation of NF-κB DNA and the transcription of IL-1a （r=0. 777,P〈0.01）. Conclusion These results suggest that IL-1a regulated by NF-κB may be a key cytokine in nasal polyps. It should he possible that the changes of microenvironment caused by NF-κB and IL-1a may play an important role in the mechanisms for the pathogenesis of nasal polyps.

关 键 词：鼻息肉 微环境 核因子-КB IL-1A 转录 

分 类 号：R765.25[医药卫生—耳鼻咽喉科]