HPLC法测定滑肠通便片中大黄素的总量  

Quantitative Determination of Emodin in Huachangtongbian Tablets by HPLC

作　　者：张健民[1] 王琦[2] 曾志坚[2] 蒋三员[1] 陈小琼[1] 

机构地区：[1]江门市新会区中医院,广东江门529100 [2]江门市药品检验所,广东江门529030

出　　处：《今日药学》2010年第4期4-5,9,共3页Pharmacy Today

基　　金：广东省中医药局资助课题(编号:2008063)

摘　　要：目的建立滑肠通便片中大黄素的含量测定方法。方法采用高效液相色谱（HPLC）法：色谱柱为迪马Diamonsil—C18（5μm，200mm×4．6mm）；检测波长：254nm；甲醇-0．1％磷酸（78：22）为流动相；进样量：20μl；流速：1．0ml／min；柱温：25℃。结果大黄素在0．08—0．8μg范围内呈良好的线性关系，r=1．0，平均加样回收率为99．0％，相对标准偏差值（RSD）=0．70％。结论本方法简便、准确，可用于滑肠通便片的质量控制。Objective To establish a method for the quantitative determination of emodin in Huachangtongbian tablets. Methods HPLC was used in the content measurement. The chromatographic separation was performed on Diamonsil-C18（5 μm, 200 mm ×4.6 mm）. The detection wavelength was 254 nm. The methanol-0.1% phosphoric acid （78： 22） was used as mobile phase. Injection volume was 20μl, and the flow was 1.0 ml/min, the column temperature was 25℃. Results The linear range of emodin was 0.08 ～0.8μg （r =1.0）. The average recovery rate was 99.0%, and RSD was 0.70%. Conclusion The method is simple, accurate, and sensitive, and can be used for the quality control of Huachangtongbian tablets.

关 键 词：HPLC 滑肠通便片 大黄素 含量测定 

分 类 号：R284.2[医药卫生—中药学]