经前平颗粒治疗经前期综合征肝气逆证大鼠的作用机制  

Mechanism of Jingqianping Granules in Treating Premenstrual Syndrome Liver-qi Invasion Model Rats

作　　者：徐明婧[1] 张惠云[1] 

机构地区：[1]山东中医药大学中医药经典理论国家教育部重点实验室,济南250355

出　　处：《药学与临床研究》2010年第2期152-155,共4页Pharmaceutical and Clinical Research

基　　金：国家自然科学基金(30930110;30973688)

摘　　要：目的:通过观察经前平颗粒对经前期综合征(PMS)肝气逆证大鼠下丘脑、顶区皮质5羟色胺和多巴胺受体亚型表达的影响,探讨经前平颗粒治疗PMS肝气逆证的机制。方法:制备PMS肝气逆症大鼠模型,经前平给药后心脏灌流4%多聚甲醛固定液,免疫组化法检测大鼠顶区皮质、下丘脑5-HT1AR、D3R阳性细胞。结果:经前平颗粒能使模型大鼠顶区皮质、下丘脑5-HT1A和D3受体阳性细胞明显增多(P<0.05)。结论:经前平颗粒可通过调节中枢5-HT1AR和D3R受体的表达,治疗PMS肝气逆证及相关的情志障碍。Objective： To approach the mechanism by observing the effects of Jingqianping granules on the expression levels of hypotypes of 5-hydroxytryptamine receptors （5-HTIAR） and dopamine receptors （D3R） in hypothalamuses and parietal region cortexes of the premenstrual syndrome （PMS） liver-qi invasion model rats. Methods： The rat model of PMS liver-qi invasion was established for the administrations of Jingqianping granules. After the treatment, the heart was perfused with 4% polyoxymethylene, and the positive cells of 5-HT1AR and D3R were checked in the hypothalamuses and parietal region cortexes by immunohistochemistry. Results： Jingqianping granules can increase the masculine cells of 5-HT1AR and D3R in the hypothalamuses and parietal region cortexes of the model rats. Conclusions： Jingqianping granules can cure premenstrual syndrome liver-qi invasion and relative affectional disorders by accommodating the central expressions of-HT1AR and D3R.

关 键 词：PMS肝气逆证 经前平颗粒 免疫组织化学 5-HT1A受体 D3受体 

分 类 号：R965.1[医药卫生—药理学]