甲氨蝶呤红细胞载体对肝癌模型大鼠肝肾功能的保护作用  

Protection of Hepatic and Renal Function by Methotrexate Loading Erythrocytes in Hepatocarcinoma Rats

作　　者：袁盛华[1] 汪浩[1] 葛卫红[1] 张尚锁[2] 

机构地区：[1]南京大学医学院附属鼓楼医院,南京210008 [2]南京中医药大学,南京210029

出　　处：《药学与临床研究》2010年第2期164-167,共4页Pharmaceutical and Clinical Research

摘　　要：目的:观察甲氨蝶呤红细胞载体(MTX-RBCs)对肝癌模型大鼠肝肾功能的保护作用,为MTX-RBCs的应用提供初步的理论和实验依据。方法:32只SD大鼠随机分为4组,分别为空白对照组、模型对照组、MTX直接静脉注射组和MTX-RBCs静脉给药组。含W256细胞的癌性腹水直接肝内注射建立大鼠肝癌模型。监测大鼠体重变化和生存时间,8天后处死大鼠,剥取肿瘤组织,留取大鼠血清样本,测定丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、血清尿素氮(BUN)及肌酐(Scr)等生化指标。结果:大鼠肝癌模型复制成功,在成瘤过程中给予MTX-RBCs,能够明显延长大鼠生存期,降低肿瘤体积(P<0.01);与模型对照组及MTX直接静脉注射组相比,MTX-RBCs静脉给药组大鼠血清ALT、AST、BUN、Scr等指标显著降低,甚至接近正常水平。结论:高渗法制备的MTX-RBCs,经静脉注射在抑制大鼠肝肿瘤形成的同时,对大鼠肝肾功能具有显著的保护作用(P<0.001),从而大大增加其安全性。Objective： To observe the hepatic and renal functions of hepatoma bearing rats after the administration of methotrexate loading erythrocytes （MTX-RBCs）. Methods： Thirty-two SD rats were randomly divided into 4 groups of the control, the model and the models with free MTX or MTX-RBCs injection. We monitored the body weight and survival time since the hepatoma model was formed by intrahepatic injection of walker 256 cells containing ascites. Eight days later, rat tumors were removed and measured. The serum samples were also kept for ALT, AST, BUN and Scr determination. Results： The hepatoma rat model was well built, and the MTX-RBCs venous injection could significantly prolong surviving time of rats with mild changes of body weights. Interestingly, compared with the free MTX injection, the MTX-RBCs injection also lowered ALT, AST, BUN and Scr levels and obviously reduced tumor volume in the same time. Conclusion： Intravenous injection of hyperosmotically prepared MTX-RBCs can inhibit tumor formation and perform well on protecting hepatic and renal function in experimental hepatoma bearing rats, thus be recognized as a safe and potential treatment.

关 键 词：甲氨蝶呤红细胞载体 肝肾功能 保护作用 肝癌 

分 类 号：R965.3[医药卫生—药理学]