PMI基因为筛选标记的抗旱表达载体构建  

CONSTURCTION OF PLANT EXPRESSION VECTOR CONTAINING DROUGHT RESISTANT GENE WITH PMI AS SELECTION MARKERS

作　　者：吴杨[1] 贺俐[1] 

机构地区：[1]井冈山大学生命科学学院,江西吉安343009

出　　处：《井冈山大学学报（自然科学版）》2010年第2期26-31,共6页Journal of Jinggangshan University (Natural Science)

基　　金：江西省教育厅科技计划项目(GJJ10202);江西省自然科学基金项目(2009GQN0077)

摘　　要：利用PCR克隆了大肠杆菌(Escherichia coli.)6-磷酸甘露糖异构酶(pmi)并进行了序列测定,序列分析表明,该片段与已经报道同源性为98.9%,推导的氨基酸序列与报道的同源性为100%。将该基因替换植物表达载体pCAMBIA130l中的潮霉素抗性基因,成功构建了以pmi为选择标记基因的植物表达载体pCAMBIA1301DP,为抗旱转基因育种奠定了物质基础。The 6-phosphomannose isomerase gene（pmi）of Escherichia coli was amplified by PCR. Sequence analysis showed that it shared 98.9% nucleotide acids identities and 100% amino acids identities with the sequences of pmi genes isolates reported in the NCBI. Based on pCAMBIA1301, the plant expression pCAMBIA1301DP was constructed successfully by substituting pmi for hygromycin resistance gene,which will lay the material foundation for plant drought-resistant transgenic breeding.

关 键 词：PMI 标记基因 植物表达载体 

分 类 号：Q782[生物学—分子生物学]