不同产地主要淫羊藿品种中活性黄酮类成分分析及资源研究  

Analysis of bioactive flavonoids and resources research of main Herba Epimedii species from different origins

作　　者：周政政[1] 罗建光[1] 郭宝林[2] 孔令义[1] 

机构地区：[1]中国药科大学天然药物化学教研室,南京210009 [2]中国医学科学院北京协和医学院药用植物研究所,北京100094

出　　处：《中国药科大学学报》2010年第2期146-150,共5页Journal of China Pharmaceutical University

基　　金：国家自然科学基金重点项目资助(No.30830116)~~

摘　　要：淫羊藿主要活性成分为黄酮类化合物。本文通过分析不同品种不同产地淫羊藿中抗骨质疏松成分总黄酮、淫羊藿苷及朝藿定C的含量,筛选出可能对骨质疏松具有较好治疗作用的质量稳定的淫羊藿品种。通过HPLC对收集自全国各地共计4个品种36个批次的淫羊藿样品中淫羊藿苷及朝藿定C的含量进行同时测定,并利用UV法测定了总黄酮的含量。柔毛淫羊藿、朝鲜淫羊藿、心叶淫羊藿、箭叶淫羊藿中淫羊藿苷和朝藿定C的含量总和平均值分别为20.83、7.61、14.43和23.29mg/g;总黄酮含量分别为48.39、47.76、51.35和59.79mg/g,不同品种含量差别显著。柔毛与箭叶淫羊藿质量均较好,但箭叶淫羊藿在市场上基本无流通且质量不稳定,而柔毛淫羊藿分布广泛、质量稳定、有效成分含量较高,可用于开发抗骨质疏松的潜在治疗药物。Herba Epimedii （named Yinyanghuo in Chinese） of the family Berberidaceaeis one of the most famous traditional Chinese medicines （TCMs） recorded in the pharmacopoeia of the People′s Republic of China.Total flavonoids of Epimedium（TFE）are considered to be its active components.TFE content was measured by ultraviolet method and its representative constituents,icariin and epimedin C,were determined by high performance liquid chromatography.The test results from 36 samples of 4 species showed that the contents differed significantly in different species.The average data of the summation （icariin and epimedin C） content were 20.83,7.61,14.43,23.29 mg/g in Epimedium pubescens Maxim.,E.koreanum Nakai.,E.brevicornum Maxim.,E.sagittatum（Sieb.Et Zucc.） Maxim.,respectively.Both E.sagittatum and E.pubescens performed better than the other two batches.E.sagittatum hardly has any circulation in Chinese herbal medicine market.The results showed that E.pubescens ,which had much more amounts of icariin,epimedin C and TFE than those of other species,has a better quality and may be considered as potential anti osteoporosis drug.

关 键 词：淫羊藿 总黄酮 淫羊藿苷 朝藿定C 

分 类 号：R284.1[医药卫生—中药学]