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作 者:李慧[1] 刘梦阳[1] 王清[1] 申黎艳[2] 赵丽萍[2]
机构地区:[1]青岛大学医学院附属医院检验科,青岛266003 [2]青岛市市立医院内科
出 处:《现代预防医学》2010年第9期1759-1761,1763,共4页Modern Preventive Medicine
摘 要:[目的]探讨ACE和AT1R基因多态性与2型糖尿病肾病的相互关系,从分子水平揭示发病机制。[方法]应用多聚酶链反应(PCR)结合限制性酶切技术,检测40例正常人和60例2型糖尿病患者ACE、AT1R的基因型和等位基因频率。[结果]①糖尿病(DM)组与糖尿病肾病(DN)组间的AT1R基因型构成和等位基因频率差异均无统计学意义(χ2=0.017,P﹥0.05;χ2=0.012,P﹥0.05)。DM组与DN组分别同对照组相比AT1R基因型构成差异均有统计学意义(χ2=8.40,P﹤0.01;χ2=7.06,P﹤0.01);等位基因频率差异也均有统计学意义(χ2=7.14,P﹤0.01;χ2=6.11,P﹤0.05);②DM组、DN组与对照组3组之间ACE基因型构成、等位基因频率差异均无统计学意义(P﹥0.05);③糖尿病肾病患者每分钟尿白蛋白排泄率与ACE和AT1R基因多态性可能无明显相关性(P﹥0.05)。[结论]ACE基因的DD基因型和D等位基因可能不是2型糖尿病及糖尿病肾病发生的危险因子;AT1R基因的C等位基因是2型糖尿病及糖尿病肾病发生的危险因子;D和C等位基因可能不是糖尿病肾病患者出现蛋白尿的危险因素。[Objective] To investigate the association between the ACE and AT1R gene polymorphisms with diabetic mellitus on molecular level, and explore related virulence gene. [Methods] The ACE and AT1R genotypes in the control group (40 subjects) and the study group (60 patients) were detected by the polymerase chain reaction (PCR). [Results] ① There was no difference in AT1R genetypes frequency and the allele frequency between the patients with type 2 diabetes with early nephropathy and the DM group (χ^2 = 0.017, P﹥0.05; χ^2 = 0.012, P﹥0.05). There were significant differences in AT1R genetypes frequency and the allele frequency between the DM group and the normal group (χ^2 = 8.40, P﹤0.01; χ^2 = 7.06, P ﹤0.01) and between the patients with type 2 diabetes with early nephropathy and the normal group (χ^2 = 7.14, P﹤0.01; χ^2 = 6.11, P﹤0.05). There was no significant difference in ACE genetypes, I / D allele frequency and DD / non DD genotype among the three groups (P﹥0.05). In the DN group, the ACE genetype and AT1R genetype did not relate with albumin excretion rate (P﹥0.05). [Conclusion] DD genotype and D allele are not the risk factors of T2DM and DN. C allele is the risk factor of T2DM and DN. D and C alleles are not the dangerous factors of albuminuria possibly.
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