蚯蚓体腔液体外抗呼吸道合胞病毒的实验研究  

Experimental research on anti-respiratory syncytial virus effect in vitro of earthworm coelomic fluid

作　　者：李方[1] 王建华[2] 张建林[1] 钟儒刚[2] 牛勃[1] 

机构地区：[1]山西医科大学生物化学与分子生物学教研室,太原030001 [2]北京工业大学生命科学与生物工程学院

出　　处：《中华实验和临床病毒学杂志》2010年第2期116-118,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：博士后科学基会项目（20090450272）

摘　　要：目的研究蚯蚓体腔液（Earthworm coelomic fluid，ECF）体外抗呼吸道合胞病毒（RSV）的作用。方法以Hep-2细胞为宿主细胞，利巴韦林作为阳性对照药物，通过观察细胞病变效应（CPE）和MTT染色法来研究蚯蚓体腔液体外抗RSV的作用。结果ECF和利巴韦林对Hep-2细胞的CC50分别为3．11mg／ml和1．35mg／ml。在直接杀灭作用实验中，IC50为184．1μg／ml，SI值为16．87；在抑制病毒复制实验中，IC50为1555．8μg／ml，SI值为1．99；在阻断病毒侵入实验中，没有观察到抗病毒的作用。结论蚯蚓体腔液具有直接杀灭和抑制RSV复制的作用，其中直接杀灭作用的抗病毒效果较明显，但不具有阻断RSV入侵的作用。Objective To explore the antivirus function in vitro of earthworm coelomic fluid（ECF） by researching its effect on inhibiting respiratory syncytial virus（RSV）. Methods By the method of Hep-2 cell culture and using ribavirin as a positive control, the anti-RSV effect of ECF was investigated by observing cytopathic effect （CPE） with MTT colorimetric assay. Results In Hep-2 ceils, the CC50 of ECF and ribavirin were 3. 11 mg/ml and 1.35 mg/ml separately. In the experiment of ECF directly killing RSV,the IC50 of ECF was 184.1 μg/ml, SI was 16.87;In the experiment of ECF preventing RSV invasion, no antiviral function of ECF within the experimental concentration range was observed; In the experiment of ECF inhibiting RSV replication,the IC50 of ECF was 1555.8 μg/ml, SI was 1.99. Conclusion ECF couldn＇t prevent virus from invading into host cell, but showed direct killing-virus function and inhibition of the virus replication.

关 键 词：寡毛目 抗病毒药 呼吸道合胞病毒 

分 类 号：R285[医药卫生—中药学]