遗传重配获得H5N1流感病毒Vero细胞适应株  

Preparation of Vero cell-adapted influenza HSN1 virus strain by genetic reassortment

作　　者：沈娟[1] 孙明波[1] 马磊[1] 宋绍辉[1] 李卫东[1] 姜述德[1] 杨净思[1] 廖国阳[1] 

机构地区：[1]中国医学科学院北京协和医学院医学生物学研究所,昆明650118

出　　处：《中华实验和临床病毒学杂志》2010年第2期119-121,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：国家863科技计划项目（2006AA02Z409）;卫生部行业科研专项（200802023）;云南省国内科技合作项目（2006YX29）

摘　　要：目的以传统遗传重配技术选育HSN1流感病毒Veto细胞适应株，制备Vero细胞H5N1流感疫苗。方法以流感病毒Vero细胞适应株A／Yunnan／1／2005Va（H3N2）为母株与反向遗传学技术改造的禽流感病毒疫苗株A／Anhui／1／2005（H5N1）共同感染SPF鸡胚和Vero细胞，用羊抗A／Yunnan／1／2005Va（H3N2）抗体筛选，血抑试验和基因测序鉴定病毒型别，并进行重配株的其他相关生物学试验。结果获得了1株在Vero细胞高产的H5N1流感病毒，重配前后的单价灭活疫苗免疫小鼠抗体血清效价差异无统计学意义（F=0．857，P〉0．05）。结论通过流感病毒Vero细胞适应株与流行株的重配和抗体筛选，可以获得H5N1流感病毒Vero细胞适应株。Objective To prepare Vero cell-adapted influenza H5N1 virus strain by Genetic Reassortment and produce influenza H5N1 vaccine using Vero cell as a substrate. Methods Ernbryonated specific pathogen-free （SPF） hen＇s eggs and Vero cells were co-infected with Vero cell-adapted influenza virus A/Yunnan/1/2005 Va（H3N2） and A/Anhui/1/2005 （ H5N1 ） via reverse genetics. The reassortant was screened with goat antibody against strain A/Yunnan/1/2005 Va（H3N2） and identified for subtype by hemagglutination-inhibition （HI） assays and gene analysis of HA and NA. Results A Vero cell-adapted influenza H5N1 virus strain was obtained, and there was no significant difference in serum antibody titers of monovalent inactivated vaccine reassorted before and after（ F = 0. 857, P 〉 0. 05）. Conelusion The Vero cell-adapted influenza virus of epidemic strain may be reassortment between Vero cell-adapted and epidemic strains.

关 键 词：流感病毒A型 重组 遗传 VERO细胞 

分 类 号：R511.7[医药卫生—内科学]