温热对HPV感染皮肤LC成熟功能的影响  

Effects of local hyperthermia on maturation of Langerhans cells in HPV-infected skin

作　　者：李晓东[1] 高兴华[1] 洪玉晓[1] 刘宇博[1] 祝立丽[1] 王雅坤[1] 王晓琴[1] 霍伟[1] 张丽[1] 陈洪铎[1] 

机构地区：[1]中国医科大学第一临床学院皮肤科暨卫生部免疫皮肤病学重点实验室,沈阳110001

出　　处：《中华实验和临床病毒学杂志》2010年第2期122-124,共3页Chinese Journal of Experimental and Clinical Virology

基　　金：教育部创新团队支持项目（IRT-0760）;国家自然科学研究基金专项基金项目（30740082）;沈阳市科学技术计划项目（1081267-9-00）;教育部博士点专项基金（20070159020）

摘　　要：目的探讨不同局部温热条件对人乳头瘤病毒（HPV）感染皮损朗格汉斯细胞（LCs）CD1a／CD83表达变化的影响，为病毒疣的治疗提供理论依据。方法采用共聚焦显微镜检测正常皮肤组织和尖锐湿疣皮损内LCCD1a／CD83的表达变化；利用37℃，42℃，45℃不同温热条件处理正常皮肤组织和尖锐湿疣皮损，采用流式分析技术检测游走至培养液中CD1a^＋／CD83^＋LC的数量变化。结果正常皮肤组织和尖锐湿疣皮损内只见CD1a^＋LC，无CD83^＋LC；正常皮肤组织和尖锐湿疣组织游走细胞中CD1a^＋／CD83^＋LC的百分比均随局部温度升高而逐渐增多。温热对尖锐湿疣组织游走LC中CD1a CD83表达影响显著高于正常皮肤组织。结论局部温热可能通过促进LC的迁移和成熟，在局部细胞免疫应答中发挥作用。Objective To investigate the possible mechanism of local hyperthermia in the treatment of warts through detecting the differences in CD1a/CD83 of Langerhans cells（LCs） in emigres from HPV- infected skin, as compared to normal skin. Methods Confocal microscopy were performed on Condyloma Accuminatum（CA） and normal skin; Freshly taken biopsies of CA and normal skin were subjected to surface heating at 37℃ , 42℃ and 45℃ respectively, for 30mins, Flow cytometry was used to determine the CD1a/ CD83 changes of LCs in emigres from CA and normal skin. Results By confocal microscopic observation, there were practically no CD1a^＋ LCs that expressed CD83 in the epidermis of both normal skin and CA. The proportions of CD1a^＋/CD83 ^＋ LCs were significantly increased with increased temperatures in emigres from both normal skin and CA. At each given temperature, the numbers of LCs in emigres from CA were greater than those from normal skin. Conclusion Local hyperthermia can promote migration and maturation of LCs in HPV-infected skin and accordingly stimulate the immune system to treat warts.

关 键 词：高热 诱发 乳头状瘤病毒 人 朗格尔汉斯细胞 树突细胞 尖锐湿疣 

分 类 号：R512.99[医药卫生—内科学]