丁香挥发油的提取工艺及化学成分分析  

Orthogonal Extraction of Clove Essential Oil with Ultrasound-assisted Method and Chemical Analysis

作　　者：付振喜[1] 王建清[1] 金政伟[1] 杨志岩[1] 

机构地区：[1]天津科技大学,天津300222

出　　处：《安徽农业科学》2010年第11期5628-5630,5637,共4页Journal of Anhui Agricultural Sciences

基　　金：国家"十一五"科技支撑计划项目(2006BAD30B02)

摘　　要：[目的]优化丁香挥发油提取工艺,为企业生产工艺改造提供依据。[方法]采用超声波辅助法从丁香中提取挥发油,以挥发油得率为指标,用正交试验L9(34)对丁香挥发油的超声提取工艺进行优选。[结果]最佳工艺为:料液比1∶20,提取时间90min,乙醇浓度75%,提取温度65℃,丁香挥发油的提取率为9%。用GC-MS技术对所提取的丁香挥发油进行成分鉴定,共分离鉴定出21种化学成分,占提取油总含量的99.867%;其中丁香酚含量为65.710%,异丁香酚含量为19.920%,石竹烯含量为9.332%。[结论]超声提取所得丁香挥发油的主要成分为丁香酚、异丁香酚和石竹烯,应用超声提取法,从丁香中提取挥发油简单易行,可操作性强。The extraction of clove essential oil by ultrasonic technology were studied.Using the yield of clove essential oil as an index,the extracting technology of essential oil from clove was studied by ultrasound-assist method L9（3^4）.The results showed that with the other same conditions,when the ethanol volume fraction was 75%,solid-liquid ratio of 1∶20,ultrasonic time of 90 min,ultrasonic temperature at 65 ℃,clove essential oil yield was up to 9%.By the application of GC-MS techniques,constituents of clove essential oil extracted were identified,and isolated 21 kinds of chemical composition covering 99.867% of the total content.The major components of clove essential oils used ultrasonic extraction was eugenol,isoeugenol and caryophyllene.Eugenol content was 65.71%; isoeugenol content was 19.92% while that of caryophyllene was 9.332%.

关 键 词：丁香挥发油 超声提取 成分鉴定 正交试验 GC-MS 

分 类 号：O629.9[理学—有机化学]