正交试验法优化黄褐毛忍冬总皂苷的提取工艺  

Optimization of the Extraction Technology of Total Saponins in Lonicera fulvotomentosa Hsu.et S.C.Cheng by Orthogonal Design

作　　者：缪艳燕[1] 张永萍[1] 

机构地区：[1]贵阳中医学院中药药剂教研室,贵州贵阳550002

出　　处：《安徽农业科学》2010年第11期5872-5873,共2页Journal of Anhui Agricultural Sciences

基　　金：贵州省中药现代化科技产业研究开发专项(黔科合社字[2009]5031号);贵州省中药现代化科技产业研究开发专项(黔科合农字[2006]5043号)

摘　　要：[目的]建立黄褐毛忍冬(Lonicera fulvotomentosa Hsu et S.C.Cheng)总皂苷的水提工艺。[方法]以黄褐毛忍冬总皂苷中有效成分α-常春藤皂苷的含量为考察指标,采用正交设计法考察加水量、煎煮时间、煎煮次数及浸泡时间4个因素对黄褐毛忍冬总皂苷提取工艺的影响,同时采用高效液相法测定α-常春藤皂苷的含量。[结果]加水量、煎煮次数、煎煮时间、浸泡时间对α-常春藤皂苷含量的影响程度不同,各因素影响程度的大小为:煎煮次数>加水量>浸泡时间>煎煮时间,即煎煮次数对煎煮条件影响最大,为最重要的因素,其次为加水量、浸泡时间、煎煮时间。最优方案为:黄褐毛忍冬药材不用浸泡,加10倍量水煎煮3次,每次1.0h。方差分析结果表明,加水量和煎煮次数对α-常春藤皂苷含量的影响具有显著意义(PA=0.034<0.05,PB=0.020<0.05),而煎煮时间、浸泡时间对水α-常春藤皂苷含量无显著影响(PC=0.500>0.05,PD=0.264>0.05),对测定结果影响小,这与直观分析结果相吻合。[结论]优选提取工艺简单、稳定、可行。[Objective] The paper aimed to optimize extraction technology for total saponins in Lonicera fulvotomentosa Hsu.et.S.C.Cheng. [Method] Active ingredient content of α-hederasaponin in total saponins in Lonicera fulvotomentosa Hsu.et.S.C.Cheng as an investigation index,Effects of four factors including water-adding amount,decocting times,decocting hours and soaking hours were explored by orthogonal design method,α- hederasaponin content of total saponins was determined by HPLC,taking α-hederin content as criterion. [Result] The order of effect factors was decocting times〉 water-adding amount 〉soaking hours 〉decocting hours,that is,decocting times had the important effect on the decocting conditions,followed by water-adding amount 〉soaking hours 〉decocting hours. The optimal solution was Lonicera fulvotomentosa Hsu.et.S.C.Cheng do not soak,plus 10 times water and decocted three times,each time 1.0 h. Analysis of variance results showed that water-adding amount and decocting times had important significance on content of α-hederasaponin （PA=0.034〈0.05,PB=0.020〈0.05）,while soaking hours and decocting hours had no significance on content of α-hederasaponin （PC=0.500〈0.05,PD=0.264〈0.05）,which was consistent with the visual analysis. [Conclusion] This optimized process is simple,stable and efficient.

关 键 词：黄褐毛忍冬 总皂苷 α-常春藤皂苷 高效液相色谱法 正交设计 

分 类 号：R284.2[医药卫生—中药学]