维生素D受体基因启动子区CDX2多态性与儿童氟斑牙的关系  

Relationship between the polymorphisms of CDX2 binding site in vitamin D receptor gene and the children′s dental fluorosis

作　　者：章润贞[1,2] 李颂[1] 潘莉[1] 岳岚 余晨晓 

机构地区：[1]安徽医科大学口腔医学院,合肥230032 [2]重要遗传病基因资源利用教育部省部共建重点实验室,合肥230032 [3]凤台县人民医院口腔科,凤台232100 [4]凤台县疾病控制中心,凤台232100

出　　处：《安徽医科大学学报》2010年第2期241-244,共4页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅重点课题(编号:KJ2008A096)

摘　　要：目的了解维生素D受体(VDR)基因启动子区CDX2结合位点多态性与儿童氟斑牙易感性的关系。方法Dean法检查8～12岁凤台县关店乡儿童氟斑牙患病情况,收集氟斑牙患者102例及正常对照者107例,采集外周静脉血提取DNA,采用等位基因特异多重聚合酶链反应(ASM-PCR)测定两组儿童VDR基因CDX2结合位点多态性。结果VDR基因CDX2结合位点多态性分布频率为:氟斑牙患者GG占48.0%、AG占25.5%、AA占26.5%;正常对照组GG占55.1%、AG占28.0%、AA占16.9%。两组基因型分布比较差异无统计学意义(P>0.05)。结论VDR基因CDX2结合位点多态性与所调查儿童的氟斑牙发生无明显关系。Objective To investigate the association of polymorphisms of CDX2 binding site in vitamin D receptor gene（VDR） with the susceptibility to children′s dental fluorosis.Methods A case-control study was conducted among children between 8 and 12 years old with （n=102） and without（n=107） dental fluorosis using Dean′s method in GuanDian countyside of FengTai county.DNA was extracted from blood samples of these children.VDR gene CDX2 polymorphisms were analyzed using allele-specific multiplex PCR（ASM-PCR）.Results The frequency distribution of CDX2 genotype was GG 48.0%,AG 25.5%,AA 26.5% in children with fluorosis,and GG 55.1%,AG 28.0%,AA 16.9% in children without fluorosis respectively.There were no significant differences in the two groups（P〈0.05）.Conclusion There are no correlation between VDR gene CDX2 polymorphisms and the children′s dental fluorosis in this study.

关 键 词：受体 骨化三醇 氟中毒 牙 多态性 单核苷酸 

分 类 号：R599.52[医药卫生—内科学] R781.2[医药卫生—临床医学]