腺苷A2A受体基因敲除小鼠瘢痕增生中TGF-β的表达及意义  

The significance of TGF-β expression in scar in adenosine receptor A_（2A） knockout mice

作　　者：肖虎[1] 冉丽[1] 禚莹莹[1] 王德昌[1] 霍然[1] 王一兵[1] 冯永强[1] 李强[1] 

机构地区：[1]山东大学附属省立医院烧伤整形科,济南250021

出　　处：《中华整形外科杂志》2010年第2期136-138,共3页Chinese Journal of Plastic Surgery

基　　金：山东省自然科学基金（2006ZRB14001）;山东省医药卫生科技发展计划青年基金（2009QZ023）;山东省优秀中青年科学家科研奖励基金（BS2009YY043）

摘　　要：目的 探讨腺苷A2A受体基因敲除小鼠在瘢痕形成中的作用及其机制.方法 4周大腺苷A2A受体基因敲除小鼠和同窝野生型小鼠各12只,制作瘢痕模型,利用HE染色观察瘢痕组织厚度、横截面积变化情况.采用比色氯胺T法测量组织羟脯氨酸含量,利用Western免疫印迹测量TGF-β表达.结果 野生型组小鼠瘢痕增生明显,其厚度、横截面积比自身对照增加倍数显著大于腺苷A2A受体基因敲除组小鼠（P〈0.01）,腺苷A2A受体基因敲除小鼠瘢痕增生轻,羟脯氨酸含量与同窝野生型组小鼠瘢痕含量相比差异有统计学（P〈0.01）,野生型组小鼠瘢痕TGF-β表达比自身对照增加倍数显著大于腺苷A2A受体基因敲除小鼠组（P〈0.01）.结论 腺苷A2A受体基因敲除小鼠瘢痕TGF-β表达降低,瘢痕增生显著减轻.Objective To discuss the mechanism of scar hypertrophy in adenosine receptor A_（2A） （A_（2A） R） knockout mice. Methods Animal models of hypertrophic scar were established in 12 A_（2A）R knockout mice and 12 wild-type mice as control. The thickness and the size of transverse section of the hypertrophic scar were observed by H-E staining. The hydroxyproline （ HYP） in the scar was measured colorimetrically. The TGF-p expression was tested by Western blotting method. Results The hypertrophic scar in wild-type mice was more severe than that in knockout mice. Compared with self-control , the increase of the thickness and the size of transverse section of hypertrophic scar was markedly higher in wild-type group than in the knockout group（ P 〈 0. 01） . There was significant difference in HYP content between the two groups （P 〈 0. 01 ）. Compared with self-control, the increase of TGF-p expression in wild-type group was much more than that in knockout group （P 〈 0. 01 ）. Conclusions The TGF-p expression decreases in the A_（2A） R knockout mice. The scar hypertrophy is also much less in the A_（2A） R knockout mice.

关 键 词：腺苷 受体 腺苷A2A 瘢痕 转化生长因子B 

分 类 号：R622[医药卫生—整形外科]