环磷酰胺对小鼠血清和局部毛囊内ICAM-1及ELAM-1水平的影响  

Effects of Cyclophosphamid on the Level of ICAM-1 and ELAM-1 In the Serum and Local Follicular of Mice

作　　者：邢飞[1] 姜波[2] 涂亚庭[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院皮肤科,湖北武汉430022 [2]湖北省襄樊市中心医院,湖北襄樊441021

出　　处：《中国皮肤性病学杂志》2010年第4期314-317,共4页The Chinese Journal of Dermatovenereology

摘　　要：目的建立C57BL/6小鼠化疗后脱发动物模型,观察化疗药物环磷酰胺对血清和毛囊局部ICAM-1和ELAM-1水平的影响。方法环磷酰胺静脉注射法建立C57BL/6小鼠化疗后的脱发模型,应用ELISA法和免疫组化法检测对照组和模型组小鼠血清及局部毛囊内ICAM-1和ELAM-1的水平。结果对照组小鼠血清中ICAM-1和ELAM-1分别为19.55±6.34ng/mL和11.45±4.96ng/mL,造模后小鼠血清中ICAM-1和ELAM-1的水平分别为50.33±9.28ng/mL和32.05±7.88ng/mL,明显高于对照组,差异有统计学意义(P<0.05)。对照组小鼠局部毛囊内ICAM-1仅少量表达,阳性率为(6.33±1.49)%,而VCAM-1几乎无表达,阳性率为(1.96±0.49)%;造模后小鼠局部毛囊内ICAM-1和ELAM-1的阳性率分别为(40.53±5.82)%和(21.09±3.14)%,与对照组相比差异有统计学意义(P<0.05)。结论环磷酰胺可能通过调节小鼠血清和局部毛囊内ICAM-1和ELAM-1的表达水平而产生化疗性脱发。Objective To investigate the effects of chemotherapy drugs on the levels of adhesion molecules ICAM-1 and ELAM-1 in the serum and local follicular of C57BL/6 mice model.Methods Using C57BL/6 mice as animal model,severe alopecia was induced by injection of cyclophosphamid （CYP） to mice.The hair growth and the histologically changes were observed in the back skin of C57BL/6 mice.The levels of ICAM-1 and ELAM-1 in the serum and local follicular were determined by ELISA and immunohistochemistry.Results The levelsof ICAM-1 and ELAM-1 in the serum were 19.55±6.34 ng/mL and 11.45±4.96 ng/mL in control team,and it were 50.33±9.28 ng/mL and 32.05±7.88 ng/mL in model team.The difference was statistically significant between the two team （P〈0.05）.The levels of ICAM-1 and ELAM-1 in local follicular of the control group were（6.33±1.49）% and（1.96±0.49）%,and in the model group were （40.53±5.82）% and （21.09±3.14）%.Both levels in model group were also obvious higher than that in the control group （P〈0.05）.Conclusions Cyclophosphamide may induced alopecia by increasing the levels of ICAM-1 and ELAM-1 in the serum and local follicular.

关 键 词：化疗后脱发 ICAM-1 ELAM-1 环磷酰胺 

分 类 号：R96[医药卫生—药理学] R758.71[医药卫生—药学]