sGC和TPO测定鉴别原发性血小板减少性紫癜和再生障碍性贫血的价值  

Clinical significance of detecting soluble glycocalicin and thrombopoietin in the differential diagnosis of idiopathic thrombocytopenic purpura and aplastic anemia

作　　者：赵益明[1] 何杨[1] 徐海燕[1] 阮长耿[1] 

机构地区：[1]苏州大学附属第一医院、江苏省血液研究所、卫生部血栓与止血重点实验室,215006

出　　处：《中华核医学杂志》2010年第2期84-86,共3页Chinese Journal of Nuclear Medicine

基　　金：基金项目：江苏省卫生厅“科教兴卫工程”临床医学中心--苏州大学附属第一医院血液病学学科开放课题（WKF07009）

摘　　要：目的探讨可溶性血小板糖盏蛋白（sGC）和血小板生成素（TPO）测定在特发性血小板减少性紫癜（ITP）和再生障碍性贫血（AA）鉴别诊断中的临床意义。方法用建立的sGC免疫放射分析法（mMA）和酶联免疫吸附测定（ELISA）法分别测83例ITP、47例AA患者及50名健康人血浆中的sGC和血清TPO水平。用方差分析Q值检验进行统计学分析，P〈0．05为差异有统计学意义。结果ITP组患者血浆sGC水平为（1024．35±232．47）μg／L，健康组为（712．50±177．11）μg／L，两者差异有统计学意义（Q=4．11，P〈0．05），而AA组患者血浆sGC为（543．56±141．28）μg／L，与健康对照组比较，差异也有统计学意义（Q=3．45，P〈0．05）；ITP组患者血清TPO为（90．32±39．43）ng／L，健康对照组为（70．29±25．16）ng／L，差异无统计学意义（Q=2．68，P〉0．05），而AA组血清TPO为（857．43±228．43）ng／L，高于ITP组和健康对照组（Q=24．45和18．25，P均〈0．01），差异有统计学意义。结论血浆sGC和血清TPO的测定有助于ITP和AA鉴别诊断及其病理机制研究。Objective To investigate the clinical significance of detecting soluble platelet glycocalicin （sGC） and thrombopoietin （TPO） in the differential diagnosis of idiopathic thrombocytopenic purpura （ITP） and aplastic anemia （AA）. Methods Plasma sGC and serum TPO in 83 patients with ITP, 47 patients with AA and 50 normal individuals were detected by immunoradiometrie assay （IRMA） and enzymelinked immunosorbent assay （ELISA） , respectively. Statistical analysis was performed using Q test and P value of 〈 0.05 was considered statistically significant. Results Plasma sGC in ITP group was significantly higher than that of normal group： （ 1024.35 ±232.47） μg/L vs （712.50 ±177.11 ） μg/L, and it was considered statistically significant （ Q = 4. 11, P 〈 0.05 ）. But plasma sGC in AA group was significantly lower than that of normal group： （543.56 ± 141.28） μg/L vs （712.50 ± 177.11 ） μg/L, which was statistically significant （ Q = 3.45, P 〈 0.05）. TPO level in ITP group was in the same range as that in normal controls ： （90.32 ± 39.43 ） ng/L vs （ 70.29 ± 25. 16 ） ng/L, which was statistically insignificant （ Q = 2.68, P 〉 0.05 ）. But serum TPO level in AA group was significantly higher than that in ITP and normal groups ： （ 857.43 ± 228.43 ） ng/L vs （90.32 ± 39.43 ） ng/L and （70.29 ± 25.16） ng/L, and they were considered statistically significant （ Q = 24.45 and 18.25, both P 〈 0.01 ）. Conclusion Detecting plasma sGC and serum TPO might be helpful for differentiating ITP and AA and for understanding the pathophysiol- ogy of thrombocytopenia.

关 键 词：紫癜 血小板减少性 贫血 再生障碍性 血小板膜糖蛋白类 血小板生成素 免疫放 射分析 酶联免疫吸附测定 

分 类 号：R55[医药卫生—血液循环系统疾病]