低氧无血清诱导乳鼠心肌细胞胰岛素样生长因子2受体表达上调  

Upregulated expression of IGF-2R is induced by hypoxia and serum deprivation in neonatal rat cardiomyocytes in vitro

作　　者：张秀芳[1] 史强[1] 崔传珏[1] 李君[1] 魏英杰[1] 胡盛寿[1] 

机构地区：[1]北京协和医学院中国医学科学院阜外心血管病医院卫生部心血管疾病再生医学重点实验室,北京100037

出　　处：《基础医学与临床》2010年第5期476-479,共4页Basic and Clinical Medicine

基　　金：科技部"863"计划课题(2006AA02Z4B8)

摘　　要：目的研究胰岛素样生长因子2受体(IGF-2R)在体外模拟心肌缺血低氧环境时表达的变化。方法分离培养SD乳鼠心肌细胞,以低氧和无血清处理不同时间点后收获细胞,用反转录聚合酶链式反应(RT-PCR)和实时定量RT-PCR测定IGF-2RmRNA的表达,用酶联免疫吸附法(ELISA)测定IGF-2R蛋白水平。结果与对照组相比,低氧无血清处理12和24 h乳鼠心肌细胞IGF-2RmRNA表达显著增高(P<0.01),处理24 h后IGF-2R蛋白表达也升高(P<0.05)。结论低氧无血清可诱导乳鼠心肌细胞IGF-2R的表达,IGF-2R可能在缺血低氧引起心肌损害过程中发挥重要的作用。Objective To investigate the expression of insulin-like growth factor 2 receptor（IGF-2R） induced by hypoxia and serum deprivation（H/SD） in cultured neonatal rat cardiomyocytes in vitro.Methods Cultured neonatal rat cardiomyocytes were randomly divided into control group and H/SD group.Expression of IGF-2R mRNA was measured by RT-PCR and real time RT-PCR.Level of IGF-2R protein was measured by enzyme-linked immunosorbent assay（ELISA）.Results Compared with control group,the expression of IGF-2R mRNA increased significantly（P〈〉0.01） in cardiomyocytes cultured in H/SD condition for 12 h and 24 h,and IGF-2R protein was upregulated in cardiomyocytes cultured in H/SD condition for 24 h（P〈0.05）.Conclusion H/SD may induce IGF-2R expression,and IGF-2R may potentially explain the mechanism of cardiomyocyte damage induced by ischemia.

关 键 词：胰岛素样生长因子2受体 低氧无血清 心肌细胞 

分 类 号：R541[医药卫生—心血管疾病]