缺氧对瘢痕疙瘩成纤维细胞影响的实验研究  

Effect of hypoxia on fibroblasts from keioid

作　　者：郑旭东[1] 高建华[1] 胡振富[1] 鲁峰[1] 钱奕炜[1] 

机构地区：[1]南方医科大学南方医院整形美容外科,广东广州510515

出　　处：《中国美容整形外科杂志》2010年第4期198-200,共3页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的 研究不同程度缺氧条件下对瘢痕疙瘩成纤维细胞的影响及其与HIF-1α基因表达的关系.方法 设立不同O2浓度组,在常氧（20%）、不同缺氧（10%、5%、1%）条件下,培养瘢痕疙瘩成纤维细胞.24 h后,应用MTT法、流式细胞仪技术和羟脯氨酸比色法,分别检测各组中成纤维细胞的活力、细胞周期和胶原合成水平.结果 不同程度缺氧各组中的瘢痕疙瘩成纤维细胞增殖均明显高于常氧对照组,且S期比例也增高;随着O2浓度的降低,成纤维细胞胶原合成水平逐渐增加.结论 缺氧条件下能增强瘢痕疙瘩成纤维细胞的增殖活力,提高胶原合成水平.这一结果 很可能是通过HIF-1α及其调控的缺氧通路产生作用.提示阻断瘢痕疙瘩内HIF-1α调控的缺氧通路有望成为预防及治疗瘢痕增生的新途径.Objective To investigate the effects of normoxia and different hypoxia on fibroblasts from keloid and relationship between expression of HIF-1α. Methods Fibroblasts from keloid cultured in vitro were placed in an incubator with different 02 concentrations at 20%, 10%, 5%, 1%. After 24 hours, the fibroblasts were collected to study. Flow cytometer and the MTT assay were performed to evaluate the division cycle and the cell proliferation of the fibroblasts. Hydroxyproline chromatometry was adopted to detect collagen synthesis of the fibroblasts. Results With the O2 concentrations decreasing, the cell proliferation and the proportion of the fibroblasts in the S stage of cell cycle also became more and more higher. When compared with the control groups, hypoxia could markedly increase collagen synthesis of the cultured fibroblasts. These results indicated a consistent tendency with the expression of HIF-1α detected in former research. Conclusion Hypoxia can induce fibroblast proliferation and increase collagen synthesis. The mechanism may be by the up-regulated expressions of HIF-1α and the hypoxia pathway induced by it subsequently. Decreasing the pathway may be advantageous to prevent scar proliferation and reduce the collagen deposition in abnormal scarring.

关 键 词：瘢痕疙瘩 成纤维细胞 缺氧 HIF-1Α 胶原合成 

分 类 号：R619.6[医药卫生—外科学]