高效的毛乳头细胞分离培养方法  

An efficient method for culturing dermal papillae cells

作　　者：焦虎[1] 刘立强[2] 范金财[2] 康宁[1] 田佳[2] 甘承[2] 冯苏云[2] 

机构地区：[1]中国医学科学院北京协和医学院整形外科医院研究中心,北京100144 [2]中国医学科学院北京协和医学院整形外科医院整形九科

出　　处：《中国美容整形外科杂志》2010年第4期212-215,共4页Chinese Journal of Aesthetic and Plastic Surgery

摘　　要：目的 寻求一种洁净且无须显微镜下操作的毛乳头分离方法 ,培养高纯度的毛乳头细胞.方法 中性蛋白酶消化头皮组织,将含有毛囊的皮下脂肪层剪下切碎,并用Ⅰ型胶原酶消化,之后通过多次离心的方法 获得毛乳头;对培养出的毛乳头细胞进行形态学观察,并检测毛乳头细胞的标志物alfa-SMA和碱性磷酸酶的表达情况.结果 通过新型的两步酶消化法可获得洁净的毛乳头,培养出的毛乳头细胞标志物检测阳性.结论 新型的两步酶消化法是一种高效低劳动强度的毛乳头分离方法.Objective To investigate an efficient method to isolate clear dermal papillaes without microscopes and culture highly purified dermal papillae cells. Methods The specimens were transected at the dermissubcutis interface after incubated with dispase solution. The upper part was discarded and the subcutaneous tissue which contained hair follicles was cut continuously into a mash. Mixed with collagenase I solution and incubated at 37 ℃ for 2.0 - 2.5 hours. Dermal papillaes were obtained by multiple centrifuges. Morphology of dermal papillae cells was doserved. Meanwhile, the expression of alfa-SMA and alkaline phosphatase were detected by immunofluorescence and specific stain. Results Clear dermal papillaes was harvested using two steps digestive treatment. The alfa-SMA and alkaline phosphatase of cells were positive. Conclusion The modified two steps digestive treatment is an efficient method for culturing dermal papillae cells and needs less labor.

关 键 词：毛乳头细胞 细胞培养 毛囊 组织工程 

分 类 号：R329.5[医药卫生—人体解剖和组织胚胎学] R318.5[医药卫生—基础医学]