聚羟基丁酸酯可吸收缝线生物相容性的实验室研究  

Investigation of the biocompatibility of polyhydroxybutyrate absorbable suture in laboratory

作　　者：伍锦华[1] 刘宾[1] 艾玉峰[2] 杨清芳[3] 董兆麟[4] 

机构地区：[1]西安市中心医院烧伤整形外科,陕西西安710003 [2]四川华美紫馨医学美容医院 [3]西北工业大学理学院应用化学系 [4]西北大学生物学系

出　　处：《中国美容医学》2010年第4期531-533,共3页Chinese Journal of Aesthetic Medicine

基　　金：陕西省科学技术研究发展计划项目(编号:2008K11-02)

摘　　要：目的:研究聚羟基丁酸酯(Polyhydroxybutyrate,PHB)可吸收缝线的生物相容性。方法:①将成纤维细胞进行体外培养,传代后与PHB可吸收缝线复合培养,设与经聚氰基丙烯酸酯处理过的PHB可吸收缝线复合培养为对照组,观察细胞生长情况;②行扫描电镜检查观察细胞在丝线上的生长情况;③以MTT法检测复合培养后的细胞活性。结果:①实验组细胞生长良好,对照组细胞漂浮,收缩成团;②扫描电镜下见细胞在丝线上贴附生长良好;③MTT法检测实验组对细胞增殖无明显影响。结论:PHB可吸收缝线具有良好的生物相容性。Objective To investigate the biocompatibility of absorbable suture made of Polyhydroxybutyrate（PHB）.Methods The fibroblast were co-cultured with PHB suture verse fibroblast co-cultured with PHB suture which had been dipped in the cyanoacrylate sdhesive（for cytotoxicity）,the proliferation and the adhering condition are observed by inverted microscope and scanning electronic microscope,Culture fibroblast in culture plates containing PHB suture,investigate the attachment rate with MTT assay.Results The number of fibroblast increased co-cultured with PHB,and the comparison cells were floated and shrinked,by scanning electronic microscope,there were cells connect each other adhering on the surface of the suture.Conclusion PHB have fine biocompatibility with fibroblast.

关 键 词：可吸收缝线 聚羟基丁酸酯 成纤维细胞 

分 类 号：Q813.1[生物学—生物工程]