芩丹胶囊对血管外膜成纤维细胞的作用机制  

Effect of Qindan Capsule on arterial adventitial fibroblasts

作　　者：任敏[1] 张继东[1] 王博[1] 刘萍[2] 姜虹[3] 刘桂林[1] 

机构地区：[1]山东大学齐鲁医院中医科,济南250012 [2]山东大学第二医院健康管理科,济南250033 [3]山东大学齐鲁医院心血管重构与功能实验室,济南250012

出　　处：《山东大学学报（医学版）》2010年第4期27-31,35,共6页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30873324)

摘　　要：目的探讨芩丹胶囊(QC)含药血清对动脉血管外膜成纤维细胞(AF)的作用机制。方法采用组织贴块法体外原代培养大鼠胸主动脉成纤维细胞,MTT法观察大[750mg/(kg·d)]、小[150mg/(kg·d)]剂量的QC含药血清对转化生长因子β1(TGF-β1)诱导的大鼠胸主动脉成纤维细胞的增殖的影响;实时定量PCR及western-blot分别检测α-平滑肌肌动蛋白(α-SMactin)和Smad3的基因及蛋白表达情况。结果大、小剂量QC含药血清呈剂量依赖性抑制TGF-β1诱导的AF的增殖(P<0.05),且用药组α-SMactin和Smad3基因及蛋白的表达较单纯刺激组显著降低(P<0.05)。结论QC含药血清通过干扰TGF-β1/Smad信号转导通路抑制AF增殖和分化,从而改善和逆转动脉血管外膜重构。Objective To investigate the effect and mechanism of the Qindan Capsule-containing serum on adventitial fibroblasts（AFs）. Methods AFs were cultured by tissue explant in vitro and induced by TGF-β1. The proliferation of AFs affected by the Qindan Capsule-containing serum with high[750 mg /（kg·d）]or low[150 mg /（kg·d）]dose was detected by MTT. Protein and mRNA expressions of α-SM actin and Smad3 were observed by Western-blot and Real-time PCR respectively. Results Both high and low doses of the Qindan Capsule-containing serum could reduce proliferation of AFs induced by TGF-β1（P〈0. 05） and inhibit expressions of mRNA and protein of α-SM actin and Smad3（P〈0. 05）. Conclusion The Qindan Capsule-containing serum has significant improved and reverse effect on aortic vascular remodeling. The mechanism may be related to inhibiting proliferation and differentiation of AFs through the TGF-β1 /Smad signaling pathway.

关 键 词：芩丹胶囊 转化生长因子Β1/SMAD 成纤维细胞 增殖 大鼠 

分 类 号：R543.5[医药卫生—心血管疾病] R285.5[医药卫生—内科学]