免疫复合物对单核巨噬细胞的调节作用  

Effects of immune complexes on monocyte-macrophages

作　　者：曲芸芸[1] 马春燕[1] 朱敏[1] 刘晓雯[1] 孙新平[1] 徐洁[1] 赵跃然[1] 

机构地区：[1]山东大学附属省立医院中心实验室,济南250021

出　　处：《山东大学学报（医学版）》2010年第4期54-58,共5页Journal of Shandong University:Health Sciences

基　　金：山东省科技攻关计划(2009GG10002008)

摘　　要：目的研究免疫复合物对单核巨噬细胞的增殖、细胞因子的分泌及吞噬功能的调节作用。方法免疫复合物组:细胞坏死上清+系统性红斑狼疮(SLE)患者血清;对照组有5组:坏死上清+正常人血清组、坏死上清组、SLE血清组、正常人血清组和培养基组,以上分别作用于U937细胞和U937细胞诱导产生的巨噬细胞。24h后,用CCK-8法检测U937细胞的增殖,RT-PCR法检测U937细胞分泌肿瘤坏死因子α(TNF-α)和B细胞活化因子(BAFF)的变化,中性红比色法检测诱导产生的巨噬细胞的吞噬功能。结果与对照组相比,免疫复合物能明显促进U937细胞的增殖(P<0.05),TNF-α和BAFF的mRNA表达水平明显增加(P<0.05),并明显抑制巨噬细胞的吞噬功能(P<0.05)。结论在免疫复合物作用下,单核巨噬细胞系统(MPS)处于异常活化状态,使免疫复合物的形成增多而清除减少,从而参与了SLE的发生发展。Objective To study the effects of immune complexes on proliferation,cytokine secretion and phagocytosis of monocyte macrophages in vitro. Methods The immune complexes group：necrosis supernatant ＋ Systemic lupus er-ythematosus（SLE） serum; five control groups： the （necrosis supernatant ＋ normal human serum） group,the necrosis supernatant group,the SLE serum group,the normal serum group and the medium group,all groups were applied to U937 cells and macrophages induced from U937 cells. 24 hours later,U937 cell proliferation was detected by CCK-8 assay,Tumor necrosis factor-a （TNF-α） and B cell activating factor （BAFF） were determined by RT-PCR,neutral red colorimetric assay phagocytosis of macrophages. Results Compared with the control groups,immune complexes could significantly promote the proliferation of U937 cells （P〈0. 05）,TNF-a and BAFF mRNA expression levels were significantly increased （P〈0. 05）,and immune complexes could significantly inhibit the phagocytosis of macrophages （P〈0. 05）. Conclusion Immune complexes can cause abnormal activation of the mononuclear phygocyte system （MPS）,which leads to increased production and decreased removal of immune complexes. Thus,immune complexes are involved in the occurrence and development of SLE.

关 键 词：红斑狼疮 免疫复合物 单核巨噬细胞 增殖 分泌 吞噬功能 

分 类 号：R446[医药卫生—诊断学]