雌二醇诱导的慢性非细菌性前列腺炎大鼠前列腺平滑肌细胞电压依赖钙离子通道的定量表达  

Quantitative expression of voltage-dependent calcium channels in prostate smooth muscle cells of rats with estradiol-induced chronic nonbacterial prostatitis

作　　者：张亮[1] 梁朝朝[1] 张贤生[1] 郝宗耀[1] 周骏[1] 樊松[1] 李玉[1] 

机构地区：[1]安徽医科大学第一附属医院泌尿外科,安徽合肥230022

出　　处：《中华男科学杂志》2010年第4期295-299,共5页National Journal of Andrology

基　　金：国家自然科学基金(30973004)~~

摘　　要：目的:建立慢性非细菌性前列腺炎大鼠模型,探讨炎症组与正常对照组大鼠前列腺平滑肌细胞电压依赖钙离子通道定量表达的差异性。方法:采用雌二醇诱导大鼠前列腺炎,体外培养纯化前列腺平滑肌细胞(PSMCs),用Trizol提取总RNA,经逆转录,SYBR Green I实时定量PCR测定各亚型钙离子通道α1亚基的mRNA相对表达水平,比较两组间的差异。结果:炎症组和对照组均见L、T、P/Q型钙通道表达,炎症组L型钙通道CaV1.2相对对照组表达量增多,分别为0.048±0.024和0.031±0.015,差异具有显著性(t=2.846,P=0.007),T、P/Q型两组间无统计学差异。结论:慢性前列腺炎PSMCs细胞膜L型钙通道CaV1.2数目上调,钙内流增多,在发病机制中可能具有一定作用。Objective：To construct a rat model of chronic nonbacterial prostatitis（CP） and investigate the difference in the quantitative expression of voltage-dependent calcium channels of prostate smooth muscle cells（PSMCs） between the models and controls.Methods：We established a CP rat model by estrogen induction,cultured and purified the PSMCs in vitro,and extracted total RNA by Trizol.Then we measured the mRNA expression of the α1 subunit in the calcium channel subtypes by reverse transcription and SYBR Green I real time RT-PCR,and compared it with that of the controls.Results：The expressions of the L-,T-and P/Q-type calcium channels were found in both the CP and control groups,and that of the CaV1.2 L-type calcium channel was significantly increased in the former as compared with the latter（0.048 ± 0.024 versus 0.031 ± 0.015,t = 2.846,P = 0.007）,but there were no statistically significant differences in the T-and P/Q-type calcium channels between the two groups.Conclusion：The number of CaV1.2 L-type calcium channels of PSMCs and calcium influx were increased in CP patients,which may be involved in the mechanism of CP.

关 键 词：前列腺炎 平滑肌 肌细胞 钙通道 聚合酶链反应 

分 类 号：R697.33[医药卫生—泌尿科学] R685.6[医药卫生—外科学]