密码优化的HPV11晚期基因在293T细胞中的表达  

Expression of codon- optimized human papilloma virus 11 (HPV 11 ) late gene in 293T cells

作　　者：袁春蓉[1] 吴剑波[1] 盛晚香[1] 严月华[1] 

机构地区：[1]武汉大学中南医院皮肤科,武汉430071

出　　处：《中国麻风皮肤病杂志》2010年第4期229-232,共4页China Journal of Leprosy and Skin Diseases

基　　金：国家自然科学基金(30700719)

摘　　要：目的:检测密码优化的HPV晚期基因在293T细胞中的表达。方法:对HPV11晚期基因进行密码优化,然后将其构建在哺乳动物细胞内质粒上。通过瞬时转染,将hL1、hL1+hL2、h(L1+L2)质粒分别导入宿主293T细胞。应用免疫组化技术,检测HPV11衣壳蛋白的存在。结果:293T.hL1细胞爬片行免疫组化染色为阳性,可见部分细胞呈棕黄着色,293T.(hLI+hL2)细胞及293T.h(L1+L2)细胞爬片行免疫组化染色为阳性,可见大量细胞均呈棕黄着色,不含HPV11晚期基因的对照组293T.EGFPC1细胞衣壳抗原免疫组化未见阳性着色。结论:经过密码优化的HPV11晚期基因可产生高表达的衣壳蛋白。Objective： To detect the expression of codonoptimized htunan papilloma virus （HPV） late gene in 293T cells. Methods： HPV11 late gene hLl ,hLl ＋ hL2 and h （L1 ＋ 1.2） was optimized, and then, was cloned into plasmids of the marmnalian cells. These vectors were transiently transfected into 293T cells. HPVI 1 capsid protein was detected in 293T cells by immunohistochemistry technicpaes. Results： 293T. hL1 cells growing on glass cover slips were positive by inununohistoehemical staining. Some cells were brown - yellow, suggestive of the expression of HPV capsid antigen. Large amount of 293T. （hLl ＋ hL2） cells and 293T. h （L1 ＋ L2）cells glowing on glass cover slips was stained in brown - yellow color, suggestive of high expression of HPV capsid antigen. The control group of 293T. EGFPCI cells without containing HPV11 late gene was negative in inununohistochemistry staining. Conclusion： Codon optimization of HPV11 late gene leads to increased gene expression in 293T cells.

关 键 词：HPV11 晚期基因 优化密码 转染 

分 类 号：Q78[生物学—分子生物学]